trpE Resolved · high auto-curated
H37Rv Rv1609 · MTBC0 mtbc0_001715 ·
516 aa · 1819867–1821417 (+) ·
RefSeq NP_216125.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | anthranilate synthase component I |
|---|---|
| MTBC0 PGAP re-annotation | anthranilate synthase component I |
| Revised (this work) | Anthranilate synthase component I. Pfam: Anth_synt_I_N (PF04715.20), Chorismate_bind (PF00425.25). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WFX3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Anthranilate synthase component 1 |
| EC (curated) |
EC 4.1.3.27
|
| Curated function | Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity). |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
H Coenzyme transport and metabolismQ Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| Preferred name | trpE |
| eggNOG description | Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine- binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia |
| Orthologous group | COG1169 |
| EC number |
EC 4.1.3.27
|
| KEGG orthology |
K01657
|
| KEGG pathways |
map00400, map00405, map01100, map01110, map01130, map01230, map02024, map02025
|
| KEGG modules |
M00023
|
| Gene Ontology (69) |
GO:0000162, GO:0000287, GO:0003674, GO:0003824, GO:0004049, GO:0005488, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005886, GO:0005950 +57 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.237 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Anth_synt_I_N | PF04715.20 | 2.0e-28 | 33–177 | Anthranilate synthase component I, N terminal region |
Chorismate_bind | PF00425.25 | 3.4e-89 | 234–492 | chorismate binding enzyme |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: trpG (anthranilate synthase component II), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0013 trpG |
anthranilate synthase component II | 999 | 1000 ctx | fusion:900 cooccurence:774 coexpression:697 experimental:788 database:900 textmining:897 |
Rv2192c trpD |
anthranilate phosphoribosyltransferase | 999 | 998 ctx | cooccurence:768 coexpression:697 experimental:778 database:900 textmining:904 |
Rv1611 trpC |
indole-3-glycerol phosphate synthase | 998 | 997 ctx | neighborhood:778 fusion:737 cooccurence:769 coexpression:653 experimental:461 textmining:656 |
Rv1613 trpA |
tryptophan synthase subunit alpha | 995 | 975 ctx | neighborhood:721 cooccurence:767 coexpression:651 textmining:818 |
Rv1612 trpB |
tryptophan synthase subunit beta | 992 | 975 ctx | neighborhood:721 cooccurence:728 coexpression:700 textmining:719 |
Rv2540c aroF |
chorismate synthase | 968 | 948 | database:900 textmining:410 |
Rv0948c |
chorismate mutase | 981 | 906 | database:900 textmining:815 |
Rv1885c |
chorismate mutase | 910 | 905 | database:900 |
Rv1610 |
membrane protein | 884 | 884 ctx | neighborhood:881 |
Rv2386c mbtI |
salicylate synthase | 872 | 857 | database:800 |
Rv1005c pabB |
para-aminobenzoate synthase component I | 856 | 856 | database:800 |
Rv3215 entC |
isochorismate synthase | 866 | 854 | database:800 |
Rv1435c hyp |
hypothetical protein | 819 | 808 | coexpression:647 experimental:461 |
Rv2949c |
chorismate pyruvate-lyase | 818 | 808 | database:800 |
Rv1608c bcpB |
peroxiredoxin | 773 | 773 ctx | neighborhood:773 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: anthranilate synthase component I
- MTBC0 PGAP product: anthranilate synthase component I
- Pfam (hmmscan --cut_ga): Anth_synt_I_N PF04715.20 (E=2e-28), Chorismate_bind PF00425.25 (E=3e-89)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216125.1)
- Domains: Pfam-A via hmmscan --cut_ga — Anth_synt_I_N (PF04715.20), Chorismate_bind (PF00425.25)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1169 - Curated reference: UniProt P9WFX3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
42 functional partner(s); context anchor
trpG - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001715|Rv1609|trpE MHADLAATTSREDFRLLAAEHRVVPVTRKVLADSETPLSAYRKLAANRPGTFLLESAENGRSWSRWSFIGAGAPTALTVREGQAVWLGAVPKDAPTGGDPLRALQVTLELLATADRQSEPGLPPLSGGMVGFFAYDMVRRLERLPERAVDDLCLPDMLLLLATDVAAVDHHEGTITLIANAVNWNGTDERVDWAYDDAVARLDVMTAALGQPLPSTVATFSRPEPRHRAQRTVEEYGAIVEYLVDQIAAGEAFQVVPSQRFEMDTDVDPIDVYRILRVTNPSPYMYLLQVPNSDGAVDFSIVGSSPEALVTVHEGWATTHPIAGTRWRGRTDDEDVLLEKELLADDKERAEHLMLVDLGRNDLGRVCTPGTVRVEDYSHIERYSHVMHLVSTVTGKLGEGRTALDAVTACFPAGTLSGAPKVRAMELIEEVEKTRRGLYGGVVGYLDFAGNADFAIAIRTALMRNGTAYVQAGGGVVADSNGSYEYNEARNKARAVLNAIAAAETLAAPGANRSGC