tesB1 Resolved · high auto-curated
H37Rv Rv1618 · MTBC0 mtbc0_001725 ·
300 aa · 1829579–1830481 (+) ·
RefSeq NP_216134.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acyl-CoA thioesterase II |
|---|---|
| MTBC0 PGAP re-annotation | acyl-CoA thioesterase II |
| Revised (this work) | Acyl-CoA thioesterase II. Pfam: 4HBT_3 (PF13622.13), Acyl_CoA_thio (PF02551.22), 4HBT_3C (PF20789.4). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06135
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable acyl-CoA thioesterase II TesB1 |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| Preferred name | tesB1 |
| eggNOG description | Acyl-CoA thioesterase |
| Orthologous group | COG1946 |
| KEGG orthology |
K10805
|
| KEGG pathways |
map01040
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.826 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
4HBT_3 | PF13622.13 | 6.2e-12 | 38–114 | Acyl-CoA thioesterase N-terminal domain |
Acyl_CoA_thio | PF02551.22 | 3.1e-48 | 154–285 | Acyl-CoA thioesterase |
4HBT_3C | PF20789.4 | 5.6e-23 | 154–285 | Acyl-CoA thioesterase C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pykA (pyruvate kinase), high confidence from genomic context alone (score 903 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2605c tesB2 |
acyl-CoA thioesterase II | 925 | 925 | database:900 |
Rv1617 pykA |
pyruvate kinase | 903 | 903 ctx | neighborhood:882 |
Rv2476c gdh |
NAD-dependent glutamate dehydrogenase | 798 | 798 | coexpression:798 |
Rv1616 hyp |
hypothetical protein | 770 | 770 ctx | neighborhood:768 |
Rv1619 hyp |
hypothetical protein | 649 | 649 ctx | neighborhood:647 |
Rv3671c marP |
serine protease | 611 | 597 | database:495 |
Rv1615 |
membrane protein | 594 | 594 ctx | neighborhood:594 |
Rv1611 trpC |
indole-3-glycerol phosphate synthase | 568 | 569 ctx | neighborhood:544 |
Rv1613 trpA |
tryptophan synthase subunit alpha | 563 | 564 ctx | neighborhood:544 |
Rv1612 trpB |
tryptophan synthase subunit beta | 580 | 554 ctx | neighborhood:544 |
Rv0154c fadE2 |
acyl-CoA dehydrogenase FadE2 | 554 | 538 | |
Rv2627c hyp |
hypothetical protein | 553 | 532 | database:460 |
Rv1614 lgt |
prolipoprotein diacylglyceryl transferase | 518 | 517 ctx | neighborhood:488 |
Rv0125 pepA |
serine protease PepA | 531 | 513 | database:495 |
Rv2613c |
AP-4-A phosphorylase | 512 | 512 | coexpression:449 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: acyl-CoA thioesterase II
- MTBC0 PGAP product: acyl-CoA thioesterase II
- Pfam (hmmscan --cut_ga): 4HBT_3 PF13622.13 (E=6e-12), Acyl_CoA_thio PF02551.22 (E=3e-48), 4HBT_3C PF20789.4 (E=6e-23)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216134.1)
- Domains: Pfam-A via hmmscan --cut_ga — 4HBT_3 (PF13622.13), Acyl_CoA_thio (PF02551.22), 4HBT_3C (PF20789.4)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1946 - Curated reference: UniProt O06135 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
57 functional partner(s); context anchor
pykA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001725|Rv1618|tesB1 MPDGKPMSDFDELLAVLDLNAVASDLFTGSHPSKNPLRTFGGQLMAQSFVASSRTLTRHHLPPSAFSVHFINGGDTAKDIEFQVIRLRDERRFANRRVDAVQDGTLLSSAMVSYMAGGRGLEHALDPPQVAEPHTRPPIGELLRGYEETVPHFVNALQPIEWRYANDPAWIMRDKGDRLAYNRVWVKALGEMPDDPVLHTATLLYSSDTTVLDSVITTHGLSWGFDRIFAASANHSVWFHRQVNFDDWVLYSTSSPVAADSRGLGSGHFFDRSGKLIATVVQEGVLKYFPATPDSAAGRS