cydC Family assigned · medium auto-curated
H37Rv Rv1620c · MTBC0 mtbc0_001727 ·
576 aa · 1831927–1833657 (-) ·
RefSeq NP_216136.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cytochrome biosyntheisis ABC transporter ATP-binding protein/permease CydC |
|---|---|
| MTBC0 PGAP re-annotation | thiol reductant ABC exporter subunit CydC |
| Revised (this work) | Thiol reductant ABC exporter subunit CydC. Pfam: ABC_tran (PF00005.34). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06137
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydC |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
V Defense mechanisms
|
|---|---|
| Preferred name | cydC |
| eggNOG description | ABC transporter |
| Orthologous group | COG1132 |
| KEGG orthology |
K06147, K06148, K16012
|
| KEGG pathways |
map02010
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.264 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ABC_tran | PF00005.34 | 1.2e-24 | 361–506 | ABC transporter |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: cydD (cytochrome biosyntheisis ABC transporter ATP-binding protein/permease CydD), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1621c cydD |
cytochrome biosyntheisis ABC transporter ATP-binding protein/permease CydD | 999 | 1000 ctx | neighborhood:882 coexpression:976 database:900 textmining:760 |
Rv1622c cydB |
cytochrome D ubiquinol oxidase subunit II CydB | 998 | 987 ctx | neighborhood:782 cooccurence:530 coexpression:884 textmining:887 |
Rv1623c cydA |
cytochrome D ubiquinol oxidase subunit I CydA | 998 | 974 ctx | neighborhood:756 cooccurence:497 coexpression:807 textmining:934 |
Rv1624c |
membrane protein | 690 | 690 ctx | neighborhood:688 |
Rv0955 |
integral membrane protein | 577 | 578 ctx | cooccurence:574 |
Rv3604c |
transmembrane protein | 478 | 478 ctx | cooccurence:478 |
Rv1859 modC |
molybdenum ABC transporter ATP-binding protein ModC | 448 | 449 ctx | cooccurence:447 |
Rv0204c |
transmembrane protein | 442 | 443 ctx | cooccurence:439 |
Rv0236c aftD |
alpha-(1->3)-arabinofuranosyltransferase | 425 | 426 ctx | cooccurence:422 |
Rv1625c cya |
adenylate cyclase | 402 | 402 | |
Rv1164 narI |
nitrate reductase subunit gamma | 403 | 332 | |
Rv1161 narG |
nitrate reductase subunit alpha | 580 | 318 | textmining:410 |
Rv1736c narX |
nitrate reductase-like protein NarX | 434 | 317 | |
Rv3147 nuoC |
NADH-quinone oxidoreductase subunit C | 400 | 189 | |
Rv0087 hycE |
formate hydrogenase HycE | 491 | 43 | textmining:490 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: cytochrome biosyntheisis ABC transporter ATP-binding protein/permease CydC
- MTBC0 PGAP product: thiol reductant ABC exporter subunit CydC
- Pfam (hmmscan --cut_ga): ABC_tran PF00005.34 (E=1e-24)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216136.1)
- Domains: Pfam-A via hmmscan --cut_ga — ABC_tran (PF00005.34)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1132 - Curated reference: UniProt O06137 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
16 functional partner(s); context anchor
cydD - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001727|Rv1620c|cydC MNRPSAVSRRQRDLLAASGLLGPRLPRILAAVALGVLSLGSALALAGVSAWLITRAWQMPPVLDLSVAVVAVRAFAISRGVLHYCERLATHDTALRAAGRARTLIYHRLAHGPAAAAVGLHSGDLAARVGADVDELANMLVRALVPIAVAAVLAVAATAVVAAVSVPAAVVLAVCLLVAGVVAPWLAGRTAAAQEAIARQHRGMRDTSAMIALEHAPELRVAGALRNVIADSQRRQHAWADALDAAARTGAIAEAMPTAAIGASLLGAVVAGIGMAPTVAPTTLAILMLLPLSAFEATVALPAAAVQLTRSRIAAARLLDLTGSNRVRETESTVSARLPVGTGVLAADVCCGHQEAQSIRVTIDLPPGARLAVTGASGAGKTTLLMTLAGLLPPVHGRVLLDGTNLSDFDEDELRSAVSFFAEDAHIFATTVRDNLLTARGDCPDDELIEALDRVGLCGWLAGLPEGLSTVLIGGAQAVSAGQRRRLLLARAVLSPARIVLLDEPVEHLDAANADLLRDLLAPNSGIMSAMRTVVVATHHLPNDIQCAELSIATDQRCRRRGTNSSDNNTNASAKT