Rv1507c Family assigned · medium auto-curated
H37Rv Rv1507c · MTBC0 mtbc0_001611 ·
231 aa · 1706536–1707231 (-) ·
RefSeq NP_216023.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | WbqC family protein |
| Revised (this work) | WbqC family protein. Pfam: WbqC (PF08889.17). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WLW5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized protein Rv1507c |
UniProt still lists this protein as Uncharacterized protein Rv1507c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversion
|
|---|---|
| eggNOG description | WbqC-like protein family |
| Orthologous group | COG0224 |
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.211 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
WbqC | PF08889.17 | 4.7e-81 | 8–224 | WbqC-like protein family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1503c (Rv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide bi), high confidence from genomic context alone (score 820 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1307 atpH |
ATP synthase subunit b/delta | 999 | 1000 | coexpression:974 experimental:999 database:650 |
Rv1310 atpD |
ATP synthase subunit beta | 985 | 984 | coexpression:729 experimental:829 database:661 |
Rv1308 atpA |
ATP synthase subunit alpha | 985 | 984 | coexpression:730 experimental:829 database:662 |
Rv1311 atpC |
ATP synthase subunit epsilon | 985 | 984 | coexpression:730 experimental:829 database:662 |
Rv1305 atpE |
ATP synthase subunit C | 985 | 983 | coexpression:730 experimental:829 database:643 |
Rv1304 atpB |
ATP synthase subunit A | 984 | 983 | coexpression:730 experimental:829 database:638 |
Rv1306 atpF |
ATP synthase subunit B | 930 | 924 | coexpression:670 experimental:773 |
Rv1506c hyp |
hypothetical protein | 914 | 878 ctx | neighborhood:479 cooccurence:761 |
Rv1505c hyp |
hypothetical protein | 887 | 864 ctx | neighborhood:443 cooccurence:752 |
Rv1503c |
Rv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide bi | 937 | 820 ctx | cooccurence:724 textmining:667 |
Rv1504c |
Rv1504c, (MTCY277.26c), len: 199 aa. Conserved hypothetical protein, similar to N-terminal region of P27833|RFFA_ECOLI lipopolysaccharide bi | 819 | 813 ctx | cooccurence:717 |
Rv0707 rpsC |
30S ribosomal protein S3 | 779 | 767 | coexpression:732 |
Rv3147 nuoC |
NADH-quinone oxidoreductase subunit C | 791 | 766 | coexpression:718 |
Rv0708 rplP |
50S ribosomal protein L16 | 789 | 763 | coexpression:730 |
Rv1502 hyp |
hypothetical protein | 762 | 761 ctx | cooccurence:688 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: WbqC family protein
- Pfam (hmmscan --cut_ga): WbqC PF08889.17 (E=5e-81)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216023.1)
- Domains: Pfam-A via hmmscan --cut_ga — WbqC (PF08889.17)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0224 - Curated reference: UniProt P9WLW5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
202 functional partner(s); context anchor
Rv1503c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001611|Rv1507c| MKKVAIVQSNYIPWRGYFDLIAFVDEFIIYDDMQYTKRDWRNRNRIKTSQGLQWITVPVQVKGRFHQKIRETLIDGTDWAKAHWRALEFNYSAAAHFAEIADWLAPIYLEEQHTNLSLLNRRLLNAICSYLGISTRLANSWDYELADGKTERLANLCQQAAATEYVSGPSARSYVDERVFDELSIRVTWFDYDGYRDYKQLWGGFEPAVSILDLLFNVGAEAPDYLRYCRQ