Rv1505c Resolved · high auto-curated
H37Rv Rv1505c · MTBC0 mtbc0_001609 ·
221 aa · 1705086–1705751 (-) ·
RefSeq NP_216021.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | acetyltransferase |
| Revised (this work) | Acetyltransferase. Pfam: Hexapep (PF00132.31), Hexapep_2 (PF14602.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P71784
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Transferase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Hexapeptide repeat of succinyl-transferase |
| Orthologous group | COG0110 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.793 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Hexapep | PF00132.31 | 1.5e-07 | 160–193 | Bacterial transferase hexapeptide (six repeats) |
Hexapep_2 | PF14602.13 | 6.3e-06 | 161–191 | Hexapeptide repeat of succinyl-transferase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1504c (Rv1504c, (MTCY277.26c), len: 199 aa. Conserved hypothetical protein, similar to N-terminal region of P27833|RFFA_ECOLI lipopolysaccharide bi), high confidence from genomic context alone (score 977 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1504c |
Rv1504c, (MTCY277.26c), len: 199 aa. Conserved hypothetical protein, similar to N-terminal region of P27833|RFFA_ECOLI lipopolysaccharide bi | 978 | 977 ctx | neighborhood:516 cooccurence:651 coexpression:875 |
Rv1506c hyp |
hypothetical protein | 974 | 947 ctx | neighborhood:781 cooccurence:626 coexpression:409 textmining:527 |
Rv1503c |
Rv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide bi | 948 | 906 ctx | neighborhood:516 cooccurence:608 coexpression:545 textmining:471 |
Rv1507c hyp |
hypothetical protein | 887 | 864 ctx | neighborhood:443 cooccurence:752 |
Rv1502 hyp |
hypothetical protein | 815 | 798 ctx | cooccurence:773 |
Rv3402c hyp |
hypothetical protein | 756 | 741 ctx | cooccurence:570 |
Rv1501 hyp |
hypothetical protein | 445 | 445 ctx | cooccurence:400 |
Rv1500 pimF |
glycosyltransferase | 453 | 428 | |
Rv1519 hyp |
hypothetical protein | 457 | 424 | |
Rv1570 bioD |
ATP-dependent dethiobiotin synthetase BioD | 430 | 401 | coexpression:401 |
Rv3556c fadA6 |
acetyl-CoA acetyltransferase FadA | 573 | 242 | textmining:461 |
Rv3523 ltp3 |
lipid carrier protein | 568 | 235 | textmining:459 |
Rv3546 fadA5 |
acetyl-CoA acetyltransferase FadA | 438 | 230 | |
Rv3225c |
GCN5-like N-acetyltransferase | 509 | 225 | |
Rv1527c pks5 |
polyketide synthase | 578 | 183 | textmining:506 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: acetyltransferase
- Pfam (hmmscan --cut_ga): Hexapep PF00132.31 (E=1e-07), Hexapep_2 PF14602.13 (E=6e-06)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216021.1)
- Domains: Pfam-A via hmmscan --cut_ga — Hexapep (PF00132.31), Hexapep_2 (PF14602.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0110 - Curated reference: UniProt P71784 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
21 functional partner(s); context anchor
Rv1504c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001609|Rv1505c| MTKPLVIFGSGDIAQLAHYYFTRDSEYEVVAFTVDRDYASVSEFCGLPLVAFDEVAQRFPPESHAMFVALAYAKLNGVRKEKYLAAKALGYELASYVSSHATVLNDGRIGENVFLLEDNTIQPFVSIGNNVTLWSGNHIGHHSTIHDHCFLASHIVVSGGVVIEEQSFIGVNATLRDHITIGSRCVVGAGALLLGDADADGVYIGTKTERRPVPSTELRKI