moeY Family assigned · medium auto-curated
H37Rv Rv1355c · MTBC0 mtbc0_001455 ·
715 aa · 1532587–1534734 (-) ·
RefSeq NP_215871.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | molybdopterin biosynthesis protein MoeY |
|---|---|
| MTBC0 PGAP re-annotation | Rv1355c family protein |
| Revised (this work) | Rv1355c family protein. Pfam: ThiF (PF00899.28). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WM11
SwissProt · reviewed
· Predicted
|
|---|---|
| UniProt name | Uncharacterized protein Rv1355c |
UniProt still lists this protein as Uncharacterized protein Rv1355c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
H Coenzyme transport and metabolism
|
|---|---|
| Preferred name | moeY |
| eggNOG description | involved in biosynthesis of a demolybdo cofactor (molybdopterin), necessary for molybdoenzymes. plays a role in the activation of the small subunit of the molybdopterin converting factor (MoaD) |
| Orthologous group | COG0476 |
| Gene Ontology (39) |
GO:0003674, GO:0003824, GO:0005575, GO:0005623, GO:0005886, GO:0006139, GO:0006396, GO:0006399, GO:0006400, GO:0006725, GO:0006807, GO:0008033 +27 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.974 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 16 missense, 1 nonsense, 1 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.74% of strains (1081) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ThiF | PF00899.28 | 5.0e-24 | 90–254 | ThiF family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1353c (HTH-type transcriptional regulator), high confidence from genomic context alone (score 953 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1354c hyp |
hypothetical protein | 990 | 973 ctx | neighborhood:779 coexpression:866 textmining:656 |
Rv1356c hyp |
hypothetical protein | 994 | 967 ctx | neighborhood:773 coexpression:860 textmining:837 |
Rv1353c |
HTH-type transcriptional regulator | 982 | 953 ctx | neighborhood:723 coexpression:839 textmining:649 |
Rv1358 |
transcriptional regulator | 870 | 871 | coexpression:852 |
Rv1357c hyp |
hypothetical protein | 848 | 848 | coexpression:848 |
Rv0895 |
diacyglycerol O-acyltransferase | 821 | 822 | coexpression:821 |
Rv3323c moaX |
MoaD-MoaE fusion protein MoaX | 838 | 815 | coexpression:446 experimental:473 |
Rv0894 |
transcriptional regulator | 734 | 734 | coexpression:734 |
Rv0417 thiG |
thiazole synthase | 741 | 720 | coexpression:647 |
Rv0416 thiS |
sulfur carrier protein ThiS | 724 | 707 | coexpression:459 experimental:463 |
Rv0866 moaE2 |
molybdopterin synthase catalytic subunit 2 | 694 | 679 | coexpression:435 |
Rv3119 moaE1 |
molybdopterin synthase catalytic subunit 1 | 694 | 678 | coexpression:434 |
Rv3025c iscS |
cysteine desulfurase | 690 | 671 | database:615 |
Rv0434 hyp |
hypothetical protein | 656 | 643 | database:595 |
Rv3724B cut5b |
Rv3724B, (MTV025.072), len: 187 aa. Probable cut5b,truncated cutinase, similar to C-terminal end of others e.g. Q9XB09|RVD2-RV1758 protein ( | 620 | 606 | database:464 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: molybdopterin biosynthesis protein MoeY
- MTBC0 PGAP product: Rv1355c family protein
- Pfam (hmmscan --cut_ga): ThiF PF00899.28 (E=5e-24)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215871.1)
- Domains: Pfam-A via hmmscan --cut_ga — ThiF (PF00899.28)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0476 - Curated reference: UniProt P9WM11 (SwissProt, reviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
73 functional partner(s); context anchor
Rv1353c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001455|Rv1355c|moeY MTIPHEGGSTGILVLRDDDHDDVLVLDRLRSDPSIEFVDRFAEQLAGVRRLLPQPDPDLLEEAKRWAYYPWRRMVVAILGPRGFRAVRLDRNRHLITAEEQRALHALRVGVVGLSAGHAIAYTLAAEGACGTLRLADFDKIELSNLNRVPVGVFDIGLNKAMIAARRIAELDPYLAVDLVTSGLSPESVDEFLDGLDVVIEECDSLDIKVILRQAACARGVPVLMATSDRGLVDVERYDVEPGRPIFHGLLGDIDADKLCGLTTKDKVPHVLNILDCQELSARCAASMIEVDQTLWGWPQLAGDIWVGAATVAEAVRRIGLGEPLESGRVRVDVSAALDRLDQPPMPSRGNGWLLESVPPTAPAEPQPTSEIVAQAAIRAPSGGNVQPWHVVAKQHSLTIRLAPEHTSAMDIAFRGSAVAVGAAMFNARVAAAAHRVLGSVEFDESQPDSPLQATMHFGRGDDPSLAALYRPMLLRTTNRHHGMPGHVHPATVELLTNTAAAEGARLQLLLSRNEIDRAATILAAADRIRYLTPRLHEEMMSELRWPGDPSLDAGIDVRSLELDSGELRVLDILRRSDVVARLAQWDCGTALEDNTNERVSASSALAIVYVDGATLTDFARGGSAMQAVWIVAQQHGLAVQPMSPIFLYARGRHDLDQASPHFAAQLHRLQLDFRELVKPGKEGHEVLIFRLFHAPPPSVCSRRRVRHAIPEPHR