thiG Resolved · high auto-curated
H37Rv Rv0417 · MTBC0 mtbc0_000437 ·
252 aa · 505731–506489 (+) ·
RefSeq NP_214931.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | thiazole synthase |
|---|---|
| MTBC0 PGAP re-annotation | thiazole synthase |
| Revised (this work) | Thiazole synthase. Pfam: ThiG (PF05690.21). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WG73
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Thiazole synthase |
| EC (curated) |
EC 2.8.1.10
|
| Curated function | Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
H Coenzyme transport and metabolism
|
|---|---|
| Preferred name | thiG |
| eggNOG description | Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S |
| Orthologous group | COG2022 |
| EC number |
EC 2.8.1.10
|
| KEGG orthology |
K03149
|
| KEGG pathways |
map00730, map01100
|
| Gene Ontology (2) |
GO:0008150, GO:0040007
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.757 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ThiG | PF05690.21 | 8.6e-108 | 8–250 | Thiazole biosynthesis protein ThiG |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: thiO (thiamine biosynthesis oxidoreductase ThiO), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0415 thiO |
thiamine biosynthesis oxidoreductase ThiO | 999 | 1000 ctx | neighborhood:881 fusion:838 cooccurence:709 coexpression:461 database:900 textmining:949 |
Rv0416 thiS |
sulfur carrier protein ThiS | 999 | 999 ctx | neighborhood:882 coexpression:873 experimental:926 textmining:828 |
Rv0414c thiE |
thiamine-phosphate synthase | 994 | 990 ctx | neighborhood:774 cooccurence:735 coexpression:852 textmining:434 |
Rv1335 cysO |
sulfur carrier protein CysO | 976 | 974 | experimental:973 |
Rv0423c thiC |
phosphomethylpyrimidine synthase | 941 | 923 ctx | cooccurence:500 coexpression:816 |
Rv3379c dxs2 |
1-deoxy-D-xylulose-5-phosphate synthase | 938 | 901 | database:900 textmining:410 |
Rv2682c dxs1 |
1-deoxy-D-xylulose 5-phosphate synthase | 963 | 900 | database:900 textmining:650 |
Rv1173 fbiC |
FO synthase | 915 | 889 | coexpression:804 experimental:413 |
Rv0422c thiD |
hydroxymethylpyrimidine/phosphomethylpyrimidine kinase | 957 | 853 ctx | cooccurence:422 coexpression:715 textmining:725 |
Rv3206c moeB1 |
adenylyltransferase/sulfurtransferase MoeZ | 880 | 836 | coexpression:729 |
Rv3116 moeB2 |
molybdenum cofactor biosynthesis protein MoeB | 879 | 835 | coexpression:729 |
Rv2338c moeW |
molybdopterin biosynthesis protein MoeW | 776 | 759 | coexpression:647 |
Rv1355c moeY |
molybdopterin biosynthesis protein MoeY | 741 | 720 | coexpression:647 |
Rv2337c hyp |
hypothetical protein | 685 | 659 | coexpression:648 |
Rv3196 hyp |
hypothetical protein | 675 | 655 | coexpression:643 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: thiazole synthase
- MTBC0 PGAP product: thiazole synthase
- Pfam (hmmscan --cut_ga): ThiG PF05690.21 (E=9e-108)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214931.1)
- Domains: Pfam-A via hmmscan --cut_ga — ThiG (PF05690.21)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2022 - Curated reference: UniProt P9WG73 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
28 functional partner(s); context anchor
thiO - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000437|Rv0417|thiG MAESKLVIGDRSFASRLIMGTGGATNLAVLEQALIASGTELTTVAIRRVDADGGTGLLDLLNRLGITPLPNTAGCRSAAEAVLTAQLAREALNTNWVKLEVIADERTLWPDAVELVRAAEQLVDDGFVVLPYTTDDPVLARRLEDTGCAAVMPLGSPIGTGLGIANPHNIEMIVAGARVPVVLDAGIGTASDAALAMELGCDAVLLASAVTRAADPPAMAAAMAAAVTAGYLARCAGRIPKRFWAQASSPAR