fabG2 Resolved · high auto-curated
H37Rv Rv1350 · MTBC0 mtbc0_001449 ·
247 aa · 1528193–1528936 (+) ·
RefSeq NP_215866.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | 3-oxoacyl-ACP reductase FabG |
|---|---|
| MTBC0 PGAP re-annotation | 3-oxoacyl-ACP reductase FabG |
| Revised (this work) | 3-oxoacyl-ACP reductase FabG. Pfam: adh_short (PF00106.32), KR (PF08659.17), adh_short_C2 (PF13561.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WGR9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized oxidoreductase Rv1350 |
| EC (curated) |
EC 1.-.-.-
|
UniProt still lists this protein as Uncharacterized oxidoreductase Rv1350; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolismQ Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| Preferred name | fabG_1 |
| eggNOG description | reductase |
| Orthologous group | COG1028 |
| EC number |
EC 1.1.1.100
|
| KEGG orthology |
K00059
|
| KEGG pathways |
map00061, map00333, map00780, map01040, map01100, map01130, map01212
|
| KEGG modules |
M00083, M00572
|
| Gene Ontology (2) |
GO:0008150, GO:0040007
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
adh_short | PF00106.32 | 1.9e-56 | 9–197 | short chain dehydrogenase |
KR | PF08659.17 | 2.5e-20 | 9–185 | KR domain |
adh_short_C2 | PF13561.13 | 2.9e-63 | 16–244 | Enoyl-(Acyl carrier protein) reductase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3538 (dehydrogenase), high confidence from genomic context alone (score 932 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2524c fas |
fatty acid synthase | 981 | 979 | coexpression:506 experimental:475 database:918 |
Rv2243 fabD |
malonyl CoA-acyl carrier protein transacylase | 957 | 956 | database:900 |
Rv3538 |
dehydrogenase | 932 | 932 ctx | fusion:900 |
Rv3389c htdY |
3-hydroxyacyl-thioester dehydratase HtdY | 931 | 931 ctx | fusion:900 |
Rv0769 |
oxidoreductase | 917 | 917 | database:900 |
Rv3559c |
oxidoreductase | 916 | 916 | database:900 |
Rv0242c fabG4 |
3-oxoacyl-ACP reductase FabG | 918 | 913 | database:900 |
Rv0649 fabD2 |
malonyl CoA-acyl carrier protein transacylase | 901 | 901 | database:900 |
Rv1351 hyp |
hypothetical protein | 776 | 776 ctx | neighborhood:773 |
Rv1349 irtB |
iron ABC transporter ATP-binding protein/permease IrtB | 698 | 689 ctx | neighborhood:528 |
Rv1348 irtA |
iron ABC transporter ATP-binding protein/permease IrtA | 692 | 678 ctx | neighborhood:506 |
Rv2246 kasB |
3-oxoacyl-ACP synthase 2 | 597 | 574 | |
Rv2245 kasA |
3-oxoacyl-ACP synthase 1 | 665 | 571 | |
Rv0484c |
short-chain type oxidoreductase | 552 | 552 ctx | cooccurence:543 |
Rv1544 |
ketoacyl reductase | 567 | 551 ctx | cooccurence:490 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: 3-oxoacyl-ACP reductase FabG
- MTBC0 PGAP product: 3-oxoacyl-ACP reductase FabG
- Pfam (hmmscan --cut_ga): adh_short PF00106.32 (E=2e-56), KR PF08659.17 (E=2e-20), adh_short_C2 PF13561.13 (E=3e-63)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215866.1)
- Domains: Pfam-A via hmmscan --cut_ga — adh_short (PF00106.32), KR (PF08659.17), adh_short_C2 (PF13561.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1028 - Curated reference: UniProt P9WGR9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
86 functional partner(s); context anchor
Rv3538 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001449|Rv1350|fabG2 MASLLNARTAVITGGAQGLGLAIGQRFVAEGARVVLGDVNLEATEVAAKRLGGDDVALAVRCDVTQADDVDILIRTAVERFGGLDVMVNNAGITRDATMRTMTEEQFDQVIAVHLKGTWNGTRLAAAIMRERKRGAIVNMSSVSGKVGMVGQTNYSAAKAGIVGMTKAAAKELAHLGIRVNAIAPGLIRSAMTEAMPQRIWDQKLAEVPMGRAGEPSEVASVAVFLASDLSSYMTGTVLDVTGGRFI