mmpL11 Resolved · high auto-curated
H37Rv Rv0202c · MTBC0 mtbc0_000216 ·
966 aa · 238744–241644 (-) ·
RefSeq NP_214716.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane transport protein MmpL11 |
|---|---|
| MTBC0 PGAP re-annotation | RND transporter MmpL11 |
| Revised (this work) | RND transporter MmpL11. Pfam: MMPL (PF03176.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJT9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Heme uptake protein MmpL11 |
| Curated function | Part of a heme-iron acquisition system. Receives heme from the heme-binding protein Rv0203 and transports it into the mycobacterial cell. Contributes to virulence..; FUNCTION: Could also transport the mycolic acid-containing lipids monomeromycolyl diacylglycerol (MMDAG) and mycolate ester wax (WE) to the bacterial surface. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | mmpL11 |
| eggNOG description | biological_process |
| Orthologous group | COG2409 |
| KEGG orthology |
K06994, K20466, K20470
|
| Gene Ontology (13) |
GO:0005575, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0016020, GO:0030312, GO:0044424, GO:0044444, GO:0044464 +1 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.578 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 10 synonymous, 16 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MMPL | PF03176.22 | 5.7e-36 | 118–369 | MMPL family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: aac (aminoglycoside 2'-N-acetyltransferase), medium confidence from genomic context alone (score 652 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0201c hyp |
hypothetical protein | 931 | 883 ctx | neighborhood:881 textmining:438 |
Rv3909 hyp |
hypothetical protein | 765 | 685 ctx | cooccurence:683 |
Rv1101c hyp |
hypothetical protein | 660 | 661 ctx | cooccurence:621 |
Rv0262c aac |
aminoglycoside 2'-N-acetyltransferase | 651 | 652 ctx | cooccurence:648 |
Rv3835 hyp |
hypothetical protein | 645 | 646 ctx | cooccurence:642 |
Rv1024 |
membrane protein | 626 | 626 ctx | cooccurence:624 |
Rv0538 |
membrane protein | 590 | 590 ctx | cooccurence:584 |
Rv0007 |
membrane protein | 534 | 520 ctx | cooccurence:512 |
Rv0203 hyp |
hypothetical protein | 875 | 512 ctx | neighborhood:507 textmining:756 |
Rv2843 hyp |
hypothetical protein | 494 | 495 ctx | cooccurence:492 |
Rv1648 |
transmembrane protein | 464 | 465 ctx | cooccurence:457 |
Rv3494c mce4F |
Mce family protein Mce4 | 442 | 443 ctx | cooccurence:435 |
Rv2164c hyp |
hypothetical protein | 440 | 441 ctx | cooccurence:435 |
Rv3658c |
transmembrane protein | 431 | 431 ctx | cooccurence:424 |
Rv1020 mfd |
transcription-repair coupling factor | 427 | 428 ctx | cooccurence:420 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane transport protein MmpL11
- MTBC0 PGAP product: RND transporter MmpL11
- Pfam (hmmscan --cut_ga): MMPL PF03176.22 (E=6e-36)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214716.1)
- Domains: Pfam-A via hmmscan --cut_ga — MMPL (PF03176.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2409 - Curated reference: UniProt P9WJT9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
42 functional partner(s); context anchor
aac - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000216|Rv0202c|mmpL11 MMRLSRNLRRCRWLVFTGWLLALVPAVYLAMTQSGNLTGGGFEVAGSQSLLVHDQLDAHYPDRGAPALALVAAPRPDASYQDIDNAVALLRQIASELPGVTEAPNPTQRPPQPDRPYVVSLRLDARNAGTSDVAKKLRDRIGVKGDQSGQTANGKVRLYVIGQGALSAAAAANTKHDIANAERWNLPIILMVLVAVFGSLAAAAIPLALAVCTVVITMGLVFVLSMHTTMSVFVTSTVSMFGIALAVDYSLFILMRYREELRCGRRPPDAVDAAMATSGLAVVLSGMTVIASLTGIYLINTPALRSMATGAILAVAVAMLTSATLTPAVLATFARAAAKRSALVHWSRRPASTQSWFWSRWVGWVMRRPWITALAASTVLLVMAAPATLMVLGNSLLRQFDSSHEIRTGAAAAAQALGPGALGPVQVLVRFDAGGASAPEHSQTIAAIRHRIAQAPNVVSVAPPRFADDNGSALLSAVLSVDPEDLGARDTITWMRTQLPRVAGAAQVDVGGPTALIKDFDDRVSATQPLVLVFVAVIAFLMLLISIRSVFLAFKGVLMTLLSVAAAYGSLVMVFQWGWARGLGFPALHSIDSTVPPLVLAMTFGLSMDYEIFLLTRIRERFLQTGQTRDAVAYGVRTSARTITSAALIMIAVFCGFAFAGMPLVAEIGVACAVAIAVDATVVRLVLVPALMAMFDRWNWWLPRWLAHILPSVDFDRPLPKVDLGDVVVIPDDFAAAIPPSADVRMVLKSAAKLKRLAPDAICVTDPLAFTGCGCDGKALDQVQLAYRNGIARAISWGQRPVHPVTVWRKRLAVALDALQTTTWECGGVQTHRAGPGYRRRSPVETTNVALPTGDRLQIPTGAETLRFKGYLIMSRNSSHDYADFADLVDTMAPETAAAVLAGMDRYYSCQAPGRQWMATQLVGRLADPQPSDLGDQSPGADAQAKWEEVRRRCLSVAVAMLEEAR