Rv1024 Family assigned · medium auto-curated
H37Rv Rv1024 · MTBC0 mtbc0_001100 ·
228 aa · 1153195–1153881 (+) ·
RefSeq NP_215540.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | membrane protein |
|---|---|
| MTBC0 PGAP re-annotation | septum formation initiator family protein |
| Revised (this work) | Septum formation initiator family protein. Pfam: DivIC (PF04977.21). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P96376
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Possible conserved membrane protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| Preferred name | divIC |
| eggNOG description | Septum formation initiator |
| Orthologous group | COG2919 |
| KEGG orthology |
K13052
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.273 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DivIC | PF04977.21 | 4.5e-19 | 86–161 | Septum formation initiator |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: eno (enolase), high confidence from genomic context alone (score 904 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2151c ftsQ |
cell division protein FtsQ | 983 | 955 | experimental:928 textmining:655 |
Rv1023 eno |
enolase | 903 | 904 ctx | neighborhood:882 |
Rv1025 hyp |
hypothetical protein | 885 | 885 ctx | neighborhood:861 |
Rv1026 ppx2 hyp |
hypothetical protein | 909 | 871 ctx | neighborhood:861 |
Rv1020 mfd |
transcription-repair coupling factor | 776 | 776 ctx | neighborhood:505 cooccurence:545 |
Rv1022 lpqU |
lipoprotein LpqU | 775 | 776 ctx | neighborhood:774 |
Rv0007 |
membrane protein | 771 | 771 ctx | cooccurence:768 |
Rv3909 hyp |
hypothetical protein | 768 | 768 ctx | cooccurence:765 |
Rv3835 hyp |
hypothetical protein | 767 | 768 ctx | cooccurence:761 |
Rv3658c |
transmembrane protein | 747 | 748 ctx | cooccurence:746 |
Rv0538 |
membrane protein | 744 | 745 ctx | cooccurence:741 |
Rv2843 hyp |
hypothetical protein | 729 | 729 ctx | cooccurence:725 |
Rv2164c hyp |
hypothetical protein | 708 | 696 ctx | cooccurence:688 |
Rv3258c hyp |
hypothetical protein | 668 | 668 ctx | cooccurence:666 |
Rv1648 |
transmembrane protein | 665 | 665 ctx | cooccurence:664 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: membrane protein
- MTBC0 PGAP product: septum formation initiator family protein
- Pfam (hmmscan --cut_ga): DivIC PF04977.21 (E=5e-19)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215540.1)
- Domains: Pfam-A via hmmscan --cut_ga — DivIC (PF04977.21)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2919 - Curated reference: UniProt P96376 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
53 functional partner(s); context anchor
eno - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001100|Rv1024| MPEAKRPESKRRSPASRPGKAGDSVRGGRATKPSAKPSTPAPHASRKTTRTPHEHIVEPIKRAITESVEKRSEQRLGFTARRAAILAAVVCVLTLTIARPVRTYFAQRAEMEQLAATEAMLRRQIADLEEQQVKLADPAYIAAQARERLGFVMPGDIPFQVQLPSTPLAPPQPGSDAATATNNEPWYTALWHTIADDPHLPPAAPPAPEPGRPGPLPPASPNPEQPGG