Rv0183 Resolved · high auto-curated
H37Rv Rv0183 · MTBC0 mtbc0_000196 ·
279 aa · 214436–215275 (+) ·
RefSeq NP_214697.2
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | lysophospholipase |
|---|---|
| MTBC0 PGAP re-annotation | monoacylglycerol lipase |
| Revised (this work) | Monoacylglycerol lipase. Pfam: Hydrolase_4 (PF12146.16), Abhydrolase_1 (PF00561.27), Abhydrolase_6 (PF12697.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O07427
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Monoacylglycerol lipase |
| EC (curated) |
EC 3.1.1.23
|
| Curated function | Involved in the hydrolysis of exogenous host lipids during chronic infection (Probable). Catalyzes the hydrolysis of both monoacylglycerols (MAG) and diacylglycerols (DAG), with a preference for MAG. It hydrolyzes 2-MAG, 1-3-MAG and MAG with short, medium and long chain fatty acids such as 1-monobutyroyl-rac-glycerol (MC4), 1-mono-octanoyl-rac-glycerol (MC8), 1-monodecanoyl-rac-glycerol (MC10), 1-monolauroyl-rac-glycerol (MC12), 1-monomyristoyl-rac-glycerol (MC14) and 1-mono-oleyl-rac-glycerol (MC18:1). Also able to hydrolyze DAG with short (DiC6) and medium (DiC10) fatty acid chains, but not . |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| Preferred name | ytpA |
| eggNOG description | hydrolase |
| Orthologous group | COG2267 |
| EC number |
EC 3.1.1.5
|
| KEGG orthology |
K01048
|
| KEGG pathways |
map00564
|
| Gene Ontology (74) |
GO:0001906, GO:0001907, GO:0003674, GO:0003824, GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0006629, GO:0008150, GO:0008152, GO:0009056 +62 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.911 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Hydrolase_4 | PF12146.16 | 3.5e-81 | 28–263 | Serine aminopeptidase, S33 |
Abhydrolase_1 | PF00561.27 | 9.3e-15 | 32–259 | alpha/beta hydrolase fold |
Abhydrolase_6 | PF12697.14 | 2.5e-15 | 33–263 | Alpha/beta hydrolase family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: sigG (ECF RNA polymerase sigma factor SigG), medium confidence from genomic context alone (score 585 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3150 nuoF |
NADH-quinone oxidoreductase subunit F | 903 | 897 | coexpression:458 experimental:817 |
Rv3151 nuoG |
NADH-quinone oxidoreductase subunit G | 897 | 888 | coexpression:415 experimental:817 |
Rv3148 nuoD |
NADH-quinone oxidoreductase subunit D | 885 | 880 | experimental:817 |
Rv3149 nuoE |
NADH-quinone oxidoreductase subunit E | 876 | 877 | coexpression:445 experimental:787 |
Rv3153 nuoI |
NADH-quinone oxidoreductase subunit I | 855 | 855 | experimental:817 |
Rv3146 nuoB |
NADH-quinone oxidoreductase subunit B | 830 | 831 | experimental:787 |
Rv3147 nuoC |
NADH-quinone oxidoreductase subunit C | 839 | 830 | experimental:817 |
Rv0184 hyp |
hypothetical protein | 822 | 822 ctx | neighborhood:821 |
Rv0185 hyp |
hypothetical protein | 822 | 822 ctx | neighborhood:821 |
Rv3152 nuoH |
NADH-quinone oxidoreductase subunit H | 804 | 805 | experimental:803 |
Rv0310c hyp |
hypothetical protein | 804 | 801 | experimental:787 |
Rv3145 nuoA |
NADH-quinone oxidoreductase subunit A | 789 | 790 | experimental:787 |
Rv3157 nuoM |
NADH-quinone oxidoreductase subunit M | 788 | 788 | experimental:787 |
Rv3158 nuoN |
NADH-quinone oxidoreductase subunit N | 732 | 732 | experimental:729 |
Rv0182c sigG |
ECF RNA polymerase sigma factor SigG | 657 | 585 ctx | neighborhood:580 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: lysophospholipase
- MTBC0 PGAP product: monoacylglycerol lipase
- Pfam (hmmscan --cut_ga): Hydrolase_4 PF12146.16 (E=3e-81), Abhydrolase_1 PF00561.27 (E=9e-15), Abhydrolase_6 PF12697.14 (E=2e-15)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214697.2)
- Domains: Pfam-A via hmmscan --cut_ga — Hydrolase_4 (PF12146.16), Abhydrolase_1 (PF00561.27), Abhydrolase_6 (PF12697.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2267 - Curated reference: UniProt O07427 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
67 functional partner(s); context anchor
sigG - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000196|Rv0183| MTTTRTERNFAGIGDVRIVYDVWTPDTAPQAVVVLAHGLGEHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKRVLVRDISEYTADFDTLVGIATREYPGCKRIVLGHSMGGGIVFAYGVERPDNYDLMVLSAPAVAAQDLVSPVVAVAAKLLGVVVPGLPVQELDFTAISRDPEVVQAYNTDPLVHHGRVPAGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVECVGSADVQLKEYPGLYHEVFNEPERNQVLDDVVAWLTERL