bglS Resolved · high auto-curated
H37Rv Rv0186 · MTBC0 - ·
691 aa · 216269–218344 (+) ·
RefSeq NP_214700.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | beta-glucosidase BglS |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Beta-glucosidase BglS. Pfam: Glyco_hydro_3 (PF00933.28), Glyco_hydro_3_C (PF01915.28), Fn3-like (PF14310.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O07430
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable beta-glucosidase BglS |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
G Carbohydrate transport and metabolism
|
|---|---|
| Preferred name | bglS |
| eggNOG description | Fibronectin type III-like domain |
| Orthologous group | COG1472 |
| EC number |
EC 3.2.1.21
|
| KEGG orthology |
K05349
|
| KEGG pathways |
map00460, map00500, map00940, map01100, map01110
|
| CAZy family |
GH3
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.348 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 12 synonymous, 11 missense, 1 nonsense, 3 frameshift |
| Disruption | 4 distinct premature-stop/frameshift site(s); most common in 0.63% of strains (916) · convergent |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Glyco_hydro_3 | PF00933.28 | 2.4e-35 | 43–292 | Glycosyl hydrolase family 3 N terminal domain |
Glyco_hydro_3_C | PF01915.28 | 5.2e-56 | 325–574 | Glycosyl hydrolase family 3 C-terminal domain |
Fn3-like | PF14310.13 | 8.5e-17 | 607–673 | Fibronectin type III-like domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: galK (galactokinase), medium confidence from genomic context alone (score 572 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2471 aglA |
alpha-glucosidase AglA | 945 | 922 | database:900 |
Rv0185 hyp |
hypothetical protein | 916 | 917 ctx | neighborhood:652 coexpression:770 |
Rv0062 celA1 |
cellulase CelA | 981 | 902 | database:900 textmining:819 |
Rv1090 celA2b |
Rv1090, (MTV017.43), len: 151 aa. Probable celA2b,second part of cellulase (endoglucanase), similar to C-terminus of others e.g. O08468 cell | 981 | 902 | database:900 textmining:819 |
Rv1089A celA2a |
Rv1089A, len: 34 aa. Probable celA2a, first part of cellulase (endoglucanase), similar to N-terminus of others. This region is a possible MT | 900 | 901 | database:900 |
Rv0184 hyp |
hypothetical protein | 713 | 713 ctx | neighborhood:652 |
Rv0620 galK |
galactokinase | 571 | 572 ctx | cooccurence:561 |
Rv3575c |
LacI family transcriptional regulator | 591 | 566 ctx | cooccurence:551 |
Rv0183 |
lysophospholipase | 557 | 537 ctx | neighborhood:537 |
Rv0729 xylB |
D-xylulose kinase XylB | 504 | 473 ctx | cooccurence:409 |
Rv0182c sigG |
ECF RNA polymerase sigma factor SigG | 437 | 437 ctx | neighborhood:419 |
Rv3242c hyp |
hypothetical protein | 436 | 437 | coexpression:433 |
Rv0181c hyp |
hypothetical protein | 400 | 401 | |
Rv0212c nadR |
transcriptional regulator NadR | 404 | 191 | |
Rv3096 hyp |
hypothetical protein | 882 | 99 | textmining:875 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): beta-glucosidase BglS
- Pfam (hmmscan --cut_ga): Glyco_hydro_3 PF00933.28 (E=2e-35), Glyco_hydro_3_C PF01915.28 (E=5e-56), Fn3-like PF14310.13 (E=9e-17)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214700.1)
- Domains: Pfam-A via hmmscan --cut_ga — Glyco_hydro_3 (PF00933.28), Glyco_hydro_3_C (PF01915.28), Fn3-like (PF14310.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1472 - Curated reference: UniProt O07430 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
21 functional partner(s); context anchor
galK - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0186|bglS MTDDERFSLLVGLTGASDLWPVRDERIPQGVPMCAGYVPGIPRLGVPALLMSDAGLGVTNPGYRPGDTATALPAGLALAASFNPVLARSSGKAIGREARSRGFNVQLAGAINLARDPRNGRNFEYLSEDPLLSATMAAESIIGIQQQGVIATTKHFSLNCNETNRHWLDAVIDPDAHRESDLLAFEIVIERSQPGAVMAAYNKVNGDYAAGNDHLLNDVLKGAWGYRGWVMSDWGGTPSWECALAGLDQECGAQIDAVLWQSEAFTDRLRAAYADGNLPKGRLSDMVRRILRSMFAVGIDRWKPAPAPDMNAHNEIAAQMARQGIVLLQNRGLLPLAPESAGRIAVIGGYAHLGVPAGYGSSAVTPPGGYAGVIPIGGSGLAAGLRNLYLLPSSPLSELRKRLPNAQFEFDPGINPAEAVLAARRADIAIVFAIRAEGEGFDSADLSLPWGQDALIAAVASANANTVVVLETGNPVTMPWRDSVNAIMQAWYPGQAGGQAVAEIVTGQVNPSGRLPITFPVDLGQTPRSQPPELGAPWGTSTTIHYTEGADVGYRWFASTNQTPMFAFGHGLSYTSFEYRDLVVTGGHTVHASFSVTNTGDRSGADVPQLYMIAAPGESRLRLLGFERVELEPGQTRRVRIEADPRLLARYDGEARSWRIEPGGYTVAVGASAVALKLAAKVKLAGRGFGR