argG Resolved · high auto-curated
H37Rv Rv1658 · MTBC0 mtbc0_001767 ·
398 aa · 1883405–1884601 (+) ·
RefSeq NP_216174.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | argininosuccinate synthase |
|---|---|
| MTBC0 PGAP re-annotation | argininosuccinate synthase |
| Revised (this work) | Argininosuccinate synthase. Pfam: Arginosuc_synth (PF00764.26), Arginosuc_syn_C (PF20979.3). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WPW7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Argininosuccinate synthase |
| EC (curated) |
EC 6.3.4.5
|
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
E Amino acid transport and metabolism
|
|---|---|
| Preferred name | argG |
| eggNOG description | Belongs to the argininosuccinate synthase family. Type 1 subfamily |
| Orthologous group | COG0137 |
| EC number |
EC 6.3.4.5
|
| KEGG orthology |
K01940
|
| KEGG pathways |
map00220, map00250, map01100, map01110, map01130, map01230, map05418
|
| KEGG modules |
M00029, M00844, M00845
|
| Gene Ontology (54) |
GO:0000050, GO:0000053, GO:0003674, GO:0003824, GO:0004055, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0006082 +42 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.126 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 16 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Arginosuc_synth | PF00764.26 | 2.0e-75 | 4–165 | Arginosuccinate synthase N-terminal HUP domain |
Arginosuc_syn_C | PF20979.3 | 4.0e-101 | 174–391 | Arginosuccinate synthase C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: argH (argininosuccinate lyase), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1659 argH |
argininosuccinate lyase | 999 | 1000 ctx | neighborhood:785 cooccurence:768 coexpression:972 database:900 textmining:804 |
Rv1656 argF |
ornithine carbamoyltransferase | 999 | 1000 ctx | neighborhood:879 coexpression:976 database:900 textmining:951 |
Rv1654 argB |
acetylglutamate kinase | 999 | 996 ctx | neighborhood:857 coexpression:973 textmining:821 |
Rv1655 argD |
acetylornithine aminotransferase | 997 | 995 ctx | neighborhood:879 coexpression:932 textmining:462 |
Rv1653 argJ |
bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase | 995 | 991 ctx | neighborhood:812 coexpression:946 textmining:551 |
Rv1657 argR |
arginine repressor | 997 | 988 ctx | neighborhood:879 coexpression:908 textmining:799 |
Rv1384 carB |
carbamoyl-phosphate synthase large subunit | 995 | 988 ctx | fusion:720 coexpression:794 database:800 textmining:651 |
Rv1652 argC |
N-acetyl-gamma-glutamyl-phoshate reductase | 995 | 984 ctx | neighborhood:812 coexpression:901 textmining:759 |
Rv0357c purA |
adenylosuccinate synthetase | 940 | 922 | database:900 |
Rv2201 asnB |
asparagine synthetase | 925 | 915 | database:900 |
Rv1538c ansA |
L-aparaginase | 924 | 907 | database:900 |
Rv1380 pyrB |
aspartate carbamoyltransferase | 912 | 907 | database:900 |
Rv1001 arcA |
arginine deiminase | 914 | 901 | database:900 |
Rv1595 nadB |
L-aspartate oxidase | 913 | 901 | database:900 |
Rv1383 carA |
carbamoyl-phosphate synthase small subunit | 919 | 895 | coexpression:456 database:800 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: argininosuccinate synthase
- MTBC0 PGAP product: argininosuccinate synthase
- Pfam (hmmscan --cut_ga): Arginosuc_synth PF00764.26 (E=2e-75), Arginosuc_syn_C PF20979.3 (E=4e-101)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216174.1)
- Domains: Pfam-A via hmmscan --cut_ga — Arginosuc_synth (PF00764.26), Arginosuc_syn_C (PF20979.3)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0137 - Curated reference: UniProt P9WPW7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
72 functional partner(s); context anchor
argH - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001767|Rv1658|argG MSERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGGEHMDVIRQRALDCGAVEAVVVDARDEFAEGYCLPTVLNNALYMDRYPLVSAISRPLIVKHLVAAAREHGGGIVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWTREKAIAFAEENAIPINVTKRSPFSIDQNVWGRAVETGFLEHLWNAPTKDIYAYTEDPTINWGVPDEVIVGFERGVPVSVDGKPVSMLAAIEELNRRAGAQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHTELEHVTLERELGRFKRQTDQRWAELVYDGLWYSPLKAALEAFVAKTQEHVSGEVRLVLHGGHIAVNGRRSAESLYDFNLATYDEGDSFDQSAARGFVYVHGLSSKLAARRDLR