Rv1930c Resolved · medium auto-curated

H37Rv Rv1930c · MTBC0 mtbc0_002044 · 174 aa · 2201034–2201558 (-) · RefSeq NP_216446.1

Annotation: from legacy to revised

Legacy (H37Rv / Mycobrowser)hypothetical protein
MTBC0 PGAP re-annotationglutamine amidotransferase
Revised (this work)Glutamine amidotransferase.

Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.

Curated reference (UniProt)

UniProt P95284 TrEMBL · unreviewed · Predicted
UniProt nameGlutamine amidotransferase

Functional vocabulary (eggNOG-mapper, orthology transfer)

COG category S Function unknown
eggNOG descriptionDJ-1 PfpI family
Orthologous groupCOG0693

Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.

Domains (Pfam, hmmscan --cut_ga)

No Pfam-A domain above the gathering threshold (or not yet scanned).

Functional interaction network (STRING v12, guilt-by-association)

Closest characterised functional partner: Rv1931c (transcriptional regulator), high confidence from genomic context alone (score 935 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.

PartnerProductScoreNo text-miningChannels (≥400)
Rv1931c transcriptional regulator 935 935 ctx neighborhood:863 cooccurence:539
Rv1929c hyp hypothetical protein 770 770 ctx neighborhood:770
Rv1932 tpx 2-Cys peroxiredoxin 763 764 ctx neighborhood:764
Rv1928c short-chain type dehydrogenase/reductase 648 647 ctx neighborhood:636
Rv0554 bpoC non-heme bromoperoxidase BpoC 596 597 ctx neighborhood:544
Rv0553 menC muconate cycloisomerase 548 548 ctx neighborhood:544
Rv0556 transmembrane protein 544 544 ctx neighborhood:544
Rv0555 menD bifunctional 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase/2-oxoglutarate decarboxylase 544 544 ctx neighborhood:544
Rv2720 lexA repressor LexA 471 448
Rv2703 sigA RNA polymerase sigma factor SigA 491 432
Rv2710 sigB RNA polymerase sigma factor SigB 491 432
Rv0517 acyltransferase 428 428 ctx cooccurence:425
Rv2043c pncA pyrazinamidase/nicotinamidase PncA 425 426 coexpression:404
Rv2511 orn oligoribonuclease 481 153 textmining:413
Rv3286c sigF RNA polymerase sigma factor SigF 484 47 textmining:481

STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.

Evidence

  • Legacy H37Rv annotation: hypothetical protein
  • MTBC0 PGAP product: glutamine amidotransferase
  • (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)

Sources

  • Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
  • Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216446.1)
  • Domains: Pfam-A via hmmscan --cut_ga — none above threshold
  • Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
  • Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021, doi:10.1093/molbev/msab293), eggNOG 5.0 DB (Huerta-Cepas et al. 2019) — OG COG0693
  • Curated reference: UniProt P95284 (TrEMBL, unreviewed; Predicted)
  • Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 16 functional partner(s); context anchor Rv1931c
  • Primary literature: none located yet; annotation rests on the domain/homology sources above.

Ancestral MTBC0 protein sequence

>mtbc0_002044|Rv1930c|
MTQIAFVAYPGVTALDVVGPYEVLRNLPHAQVRFVWLRGRRATSHWLTLPALKAFGAIPVADERIVHQDNIVTSAGVSAGLDLALWLAGQLGGEARAKAIQLAIEYDPQPPFDSGHMSKASPTTKAAATALLSKDSAKPANLTAATLLAWERALAAVQSRRRKRQPVGAQARRP