Rv0517 Resolved · high auto-curated
H37Rv Rv0517 · MTBC0 - ·
436 aa · 608746–610056 (+) ·
RefSeq NP_215031.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Acyltransferase. Pfam: Acyl_transf_3 (PF01757.29). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O33362
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Possible membrane acyltransferase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Acyltransferase |
| Orthologous group | COG1835 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.574 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 4 missense, 1 nonsense, 1 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.16% of strains (237) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acyl_transf_3 | PF01757.29 | 5.4e-26 | 37–387 | Acyltransferase domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3779 (transmembrane protein), high confidence from genomic context alone (score 738 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0518 hyp |
hypothetical protein | 912 | 909 ctx | neighborhood:757 cooccurence:640 |
Rv3779 |
transmembrane protein | 737 | 738 ctx | cooccurence:734 |
Rv0048c |
membrane protein | 737 | 738 ctx | cooccurence:734 |
Rv3700c egtE |
pyridoxal-phosphate-dependent protein EgtE | 572 | 572 ctx | cooccurence:567 |
Rv0779c |
transmembrane protein | 559 | 559 ctx | cooccurence:557 |
Rv3707c hyp |
hypothetical protein | 536 | 537 ctx | cooccurence:534 |
Rv0875c hyp |
hypothetical protein | 530 | 530 ctx | cooccurence:530 |
Rv2828c hyp |
hypothetical protein | 526 | 526 ctx | cooccurence:526 |
Rv0516c oprA |
anti-anti-sigma factor | 526 | 526 ctx | neighborhood:494 |
Rv2825c hyp |
hypothetical protein | 513 | 513 ctx | cooccurence:513 |
Rv3906c hyp |
hypothetical protein | 508 | 508 ctx | cooccurence:507 |
Rv2905 lppW |
lipoprotein LppW | 501 | 501 ctx | cooccurence:501 |
Rv3435c |
transmembrane protein | 501 | 501 ctx | cooccurence:498 |
Rv3773c hyp |
hypothetical protein | 497 | 498 ctx | cooccurence:496 |
Rv3202c adnA |
ATP-dependent DNA helicase | 492 | 492 ctx | cooccurence:476 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): acyltransferase
- Pfam (hmmscan --cut_ga): Acyl_transf_3 PF01757.29 (E=5e-26)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215031.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acyl_transf_3 (PF01757.29)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1835 - Curated reference: UniProt O33362 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
39 functional partner(s); context anchor
Rv3779 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0517| MAGGMDQPPGQPRRRTRQQSSDGKNGVRAAEITGEIRALTGLRIVAAVWVVLFHFRPMLGDASPGFRDALAPVLDCGAQGVDLFFILSGFVLTWNYLDRMGRSWSVRANLHFLWLRLARVWPVYLVTLHLAAVWVIFTLHVGHVPSPEAGQLTAISYVRQILLVQLWFQPYFDGSSWDGPAWSISAEWLAYLLFGLLILVIFRMKHATRARGLMWLAFAASLPPVVLLLASGQFYTPWSWLPRIVTQFAAGALACAAVRRLRPTDRARRIAGYLSVLVGVAIVGILYLLHAHPLAGVEDSGGVVDVLFVPLVISLAIGVGSLPALLSTRLMVFGGQISFCLYMVHELVHTAWGWAVQQYELALQDQPWKWNVVGLLAIALGAAILLYHFVEEPGRRWMRRMVDVKAASARSEPGEPVGSTRYQIDDALEGVSARAV