eccE5 Family assigned · medium auto-curated
H37Rv Rv1797 · MTBC0 mtbc0_001910 ·
406 aa · 2053494–2054714 (+) ·
RefSeq NP_216313.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | ESX-5 type VII secretion system protein EccE |
|---|---|
| MTBC0 PGAP re-annotation | type VII secretion system ESX-5 subunit EccE5 |
| Revised (this work) | Type VII secretion system ESX-5 subunit EccE5. Pfam: EccE (PF11203.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJE3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | ESX-5 secretion system protein EccE5 |
| Curated function | Part of the ESX-5 specialized secretion system, which is responsible for the secretion of EsxN and a number of PE_PGRS and PPE proteins, including PPE41. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | eccE5 |
| eggNOG description | Putative type VII ESX secretion system translocon, EccE |
| Orthologous group | 2B6RD |
| Gene Ontology (11) |
GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0016020, GO:0044424, GO:0044444, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.492 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
EccE | PF11203.14 | 1.5e-25 | 202–292 | Putative type VII ESX secretion system translocon, EccE |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mycP5 (membrane-anchored mycosin MycP), high confidence from genomic context alone (score 973 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1796 mycP5 |
membrane-anchored mycosin MycP | 987 | 973 ctx | neighborhood:881 coexpression:785 textmining:541 |
Rv1795 eccD5 |
ESX-5 type VII secretion system protein EccD | 999 | 970 ctx | neighborhood:881 experimental:652 textmining:983 |
Rv1798 eccA5 |
ESX-5 type VII secretion system protein EccA | 951 | 939 ctx | neighborhood:781 coexpression:732 |
Rv3806c ubiA |
decaprenyl-phosphate phosphoribosyltransferase | 797 | 797 | coexpression:797 |
Rv0176 |
Mce associated transmembrane protein | 797 | 797 | coexpression:797 |
Rv2553c mltG |
membrane protein | 778 | 778 | coexpression:778 |
Rv1297 rho |
transcription termination factor Rho | 754 | 754 | coexpression:754 |
Rv0006 gyrA |
DNA gyrase subunit A | 746 | 746 | coexpression:746 |
Rv0513 |
transmembrane protein | 735 | 735 | coexpression:735 |
Rv0178 |
Mce associated membrane protein | 703 | 703 | coexpression:703 |
Rv1794 espG5 hyp |
hypothetical protein | 807 | 693 ctx | neighborhood:683 textmining:400 |
Rv3870 eccCa1 |
ESX-1 secretion system protein EccCa | 673 | 413 | textmining:466 |
Rv3871 eccCb1 |
ESX-1 secretion system protein EccCb | 578 | 408 | |
Rv3894c eccC2 |
ESX-2 type VII secretion system protein EccC | 693 | 392 | textmining:516 |
Rv0284 eccC3 |
ESX-3 secretion system protein EccC3 | 465 | 384 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: ESX-5 type VII secretion system protein EccE
- MTBC0 PGAP product: type VII secretion system ESX-5 subunit EccE5
- Pfam (hmmscan --cut_ga): EccE PF11203.14 (E=1e-25)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216313.1)
- Domains: Pfam-A via hmmscan --cut_ga — EccE (PF11203.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2B6RD - Curated reference: UniProt P9WJE3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
32 functional partner(s); context anchor
mycP5 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001910|Rv1797|eccE5 MKAQRSFGLALSWPRVTAVFLVDVLILAVASHCPDSWQADHHVAWWVGVGVAAVVTLLSVVSYHGITVISGLATWVRDWSADPGTTLGAGCTPAIDHQRRFGRDTVGVREYNGRLVSVIEVTCGESGPSGRHWHRKSPVPMLPVVAVADGLRQFDIHLDGIDIVSVLVRGGVDAAKASASLQEWEPQGWKSEERAGDRTVADRRRTWLVLRMNPQRNVAAVACRDSLASTLVAATERLVQDLDGQSCAARPVTADELTEVDSAVLADLEPTWSRPGWRHLKHFNGYATSFWVTPSDITSETLDELCLPDSPEVGTTVVTVRLTTRVGSPALSAWVRYHSDTRLPKEVAAGLNRLTGRQLAAVRASLPAPTHRPLLVIPSRNLRDHDELVLPVGQELEHATSSFVGQ