Rv1794 Family assigned · medium auto-curated
H37Rv Rv1794 · MTBC0 mtbc0_001907 ·
300 aa · 2049077–2049979 (+) ·
RefSeq NP_216310.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | ESX secretion-associated protein EspG |
| Revised (this work) | ESX secretion-associated protein EspG. Pfam: ESX-1_EspG (PF14011.12). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O53943
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | ESX-5 secretion-associated protein EspG5 |
| Curated function | Specific chaperone for cognate PE/PPE proteins. Plays an important role in preventing aggregation of PE/PPE dimers. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | espG5 |
| eggNOG description | EspG family |
| Orthologous group | 2ANCJ |
| Gene Ontology (8) |
GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0016020, GO:0030312, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.198 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ESX-1_EspG | PF14011.12 | 3.0e-32 | 14–282 | EspG family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: eccD5 (ESX-5 type VII secretion system protein EccD), high confidence from genomic context alone (score 734 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1039c PPE15 |
PPE family protein PPE15 | 999 | 999 | experimental:999 |
Rv2430c PPE41 |
PPE family protein PPE41 | 999 | 999 | experimental:999 textmining:880 |
Rv2431c PE25 |
PE family protein PE25 | 967 | 898 | experimental:898 textmining:692 |
Rv1040c PE8 |
PE family protein PE8 | 898 | 898 | experimental:898 |
Rv2768c PPE43 |
PPE family protein PPE43 | 891 | 892 | experimental:891 |
Rv2770c PPE44 |
PPE family protein PPE44 | 891 | 891 | experimental:891 |
Rv1795 eccD5 |
ESX-5 type VII secretion system protein EccD | 886 | 734 ctx | neighborhood:721 textmining:591 |
Rv1796 mycP5 |
membrane-anchored mycosin MycP | 836 | 734 ctx | neighborhood:721 textmining:411 |
Rv1797 eccE5 |
ESX-5 type VII secretion system protein EccE | 807 | 693 ctx | neighborhood:683 textmining:400 |
Rv1793 esxN |
ESAT-6 like protein EsxN | 787 | 577 ctx | neighborhood:567 textmining:519 |
Rv1798 eccA5 |
ESX-5 type VII secretion system protein EccA | 727 | 422 ctx | neighborhood:415 textmining:547 |
Rv1196 PPE18 |
PPE family protein PPE18 | 463 | 309 | |
Rv2352c PPE38 |
PPE family protein PPE38 | 571 | 301 | textmining:412 |
Rv3873 PPE68 |
PPE family protein PPE68 | 484 | 298 | |
Rv1787 PPE25 |
PPE family protein PPE25 | 640 | 295 | textmining:511 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: ESX secretion-associated protein EspG
- Pfam (hmmscan --cut_ga): ESX-1_EspG PF14011.12 (E=3e-32)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216310.1)
- Domains: Pfam-A via hmmscan --cut_ga — ESX-1_EspG (PF14011.12)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2ANCJ - Curated reference: UniProt O53943 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
23 functional partner(s); context anchor
eccD5 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001907|Rv1794| MDQQSTRTDITVNVDGFWMLQALLDIRHVAPELRCRPYVSTDSNDWLNEHPGMAVMREQGIVVNDAVNEQVAARMKVLAAPDLEVVALLSRGKLLYGVIDDENQPPGSRDIPDNEFRVVLARRGQHWVSAVRVGNDITVDDVTVSDSASIAALVMDGLESIHHADPAAINAVNVPMEEMLEATKSWQESGFNVFSGGDLRRMGISAATVAALGQALSDPAAEVAVYARQYRDDAKGPSASVLSLKDGSGGRIALYQQARTAGSGEAWLAICPATPQLVQVGVKTVLDTLPYGEWKTHSRV