eccA5 Family assigned · medium auto-curated
H37Rv Rv1798 · MTBC0 mtbc0_001911 ·
610 aa · 2054711–2056543 (+) ·
RefSeq NP_216314.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | ESX-5 type VII secretion system protein EccA |
|---|---|
| MTBC0 PGAP re-annotation | type VII secretion system ESX-5 AAA family ATPase EccA5 |
| Revised (this work) | Type VII secretion system ESX-5 AAA family ATPase EccA5. Pfam: T7SS_EccA1_N (PF21545.4), AAA_5 (PF07728.21), AAA (PF00004.36), AAA_lid_6 (PF17866.9). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WPI1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | ESX-5 secretion system protein EccA5 |
| Curated function | Part of the ESX-5 specialized secretion system, which is responsible for the secretion of EsxN and a number of PE_PGRS and PPE proteins, including PPE41. EccA5 exhibits ATPase activity and may provide energy for the export of ESX-5 substrates (By similarity). |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
O Post-translational modification, protein turnover, chaperones
|
|---|---|
| Preferred name | eccA5 |
| eggNOG description | Type VII secretion |
| Orthologous group | COG0457 |
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
T7SS_EccA1_N | PF21545.4 | 3.7e-92 | 17–288 | T7SS, ESX-1 secretion system protein EccA1, N-terminal domain |
AAA_5 | PF07728.21 | 1.4e-06 | 352–426 | AAA domain (dynein-related subfamily) |
AAA | PF00004.36 | 8.4e-16 | 354–486 | ATPase family associated with various cellular activities (AAA) |
AAA_lid_6 | PF17866.9 | 4.9e-07 | 490–597 | AAA lid domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mycP5 (membrane-anchored mycosin MycP), high confidence from genomic context alone (score 949 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1796 mycP5 |
membrane-anchored mycosin MycP | 970 | 949 ctx | neighborhood:780 coexpression:732 textmining:439 |
Rv1797 eccE5 |
ESX-5 type VII secretion system protein EccE | 951 | 939 ctx | neighborhood:781 coexpression:732 |
Rv1795 eccD5 |
ESX-5 type VII secretion system protein EccD | 931 | 844 ctx | neighborhood:780 textmining:579 |
Rv3887c eccD2 |
ESX-2 secretion system protein EccD | 509 | 496 ctx | cooccurence:493 |
Rv3805c aftB |
terminal beta-(1->2)-arabinofuranosyltransferase | 474 | 474 | coexpression:474 |
Rv0256c PPE2 |
PPE family protein PPE2 | 462 | 463 ctx | cooccurence:461 |
Rv1794 espG5 hyp |
hypothetical protein | 727 | 422 ctx | neighborhood:415 textmining:547 |
Rv2574 hyp |
hypothetical protein | 409 | 409 ctx | cooccurence:409 |
Rv3895c eccB2 |
ESX-2 secretion system protein EccB | 774 | 383 | textmining:650 |
Rv3894c eccC2 |
ESX-2 type VII secretion system protein EccC | 502 | 375 | |
Rv1793 esxN |
ESAT-6 like protein EsxN | 524 | 321 | |
Rv3884c eccA2 |
ESX-2 secretion system protein EccA | 428 | 284 | |
Rv1782 eccB5 |
ESX-5 type VII secretion system protein EccB5 | 848 | 261 | textmining:804 |
Rv1783 eccC5 |
ESX-5 type VII secretion system protein EccC5 | 595 | 209 | textmining:510 |
Rv3883c mycP1 |
membrane-anchored mycosin | 456 | 80 | textmining:434 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: ESX-5 type VII secretion system protein EccA
- MTBC0 PGAP product: type VII secretion system ESX-5 AAA family ATPase EccA5
- Pfam (hmmscan --cut_ga): T7SS_EccA1_N PF21545.4 (E=4e-92), AAA_5 PF07728.21 (E=1e-06), AAA PF00004.36 (E=8e-16), AAA_lid_6 PF17866.9 (E=5e-07)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216314.1)
- Domains: Pfam-A via hmmscan --cut_ga — T7SS_EccA1_N (PF21545.4), AAA_5 (PF07728.21), AAA (PF00004.36), AAA_lid_6 (PF17866.9)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0457 - Curated reference: UniProt P9WPI1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
22 functional partner(s); context anchor
mycP5 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001911|Rv1798|eccA5 MTRPQAAAEDARNAMVAGLLASGISVNGLQPSHNPQVAAQMFTTATRLDPKMCDAWLARLLAGDQSIEVLAGAWAAVRTFGWETRRLGVTDLQFRPEVSDGLFLRLAITSVDSLACAYAAVLAEAKRYQEAAELLDATDPRHPFDAELVSYVRGVLYFRTKRWPDVLAQFPEATQWRHPELKAAGAAMATTALASLGVFEEAFRRAQEAIEGDRVPGAANIALYTQGMCLRHVGREEEAVELLRRVYSRDAKFTPAREALDNPNFRLILTDPETIEARTDPWDPDSAPTRAQTEAARHAEMAAKYLAEGDAELNAMLGMEQAKKEIKLIKSTTKVNLARAKMGLPVPVTSRHTLLLGPPGTGKTSVARAFTKQLCGLTVLRKPLVVETSRTKLLGRYMADAEKNTEEMLEGALGGAVFFDEMHTLHEKGYSQGDPYGNAIINTLLLYMENHRDELVVFGAGYAKAMEKMLEVNQGLRRRFSTVIEFFSYTPQELIALTQLMGRENEDVITEEESQVLLPSYTKFYMEQSYSEDGDLIRGIDLLGNAGFVRNVVEKARDHRSFRLDDEDLDAVLASDLTEFSEDQLRRFKELTREDLAEGLRAAVAEKKTK