Rv0874c Family assigned · medium auto-curated
H37Rv Rv0874c · MTBC0 mtbc0_000929 ·
386 aa · 975758–976918 (-) ·
RefSeq NP_215389.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | FIST C-terminal domain-containing protein |
| Revised (this work) | FIST C-terminal domain-containing protein. Pfam: FIST (PF08495.16), FIST_C (PF10442.15). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WKR9
SwissProt · reviewed
· Predicted
|
|---|---|
| UniProt name | Uncharacterized protein Rv0874c |
UniProt still lists this protein as Uncharacterized protein Rv0874c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | FIST C domain |
| Orthologous group | COG4398 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.191 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
FIST | PF08495.16 | 3.5e-24 | 35–219 | FIST N domain |
FIST_C | PF10442.15 | 1.7e-24 | 222–364 | FIST C domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0876c (transmembrane protein), medium confidence from genomic context alone (score 568 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0876c |
transmembrane protein | 568 | 568 ctx | neighborhood:544 |
Rv0875c hyp |
hypothetical protein | 555 | 555 ctx | neighborhood:544 |
Rv2671 ribD |
bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase | 444 | 152 | |
Rv0842 |
integral membrane protein | 718 | 55 | textmining:714 |
Rv1898 hyp |
hypothetical protein | 805 | 54 | textmining:803 |
Rv2672 |
protease | 640 | 47 | textmining:638 |
Rv2724c fadE20 |
acyl-CoA dehydrogenase FadE20 | 807 | 46 | textmining:806 |
Rv0552 hyp |
hypothetical protein | 806 | 45 | textmining:806 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: FIST C-terminal domain-containing protein
- Pfam (hmmscan --cut_ga): FIST PF08495.16 (E=3e-24), FIST_C PF10442.15 (E=2e-24)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215389.1)
- Domains: Pfam-A via hmmscan --cut_ga — FIST (PF08495.16), FIST_C (PF10442.15)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4398 - Curated reference: UniProt P9WKR9 (SwissProt, reviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
8 functional partner(s); context anchor
Rv0876c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000929|Rv0874c| MRIGVGVCTTPDARQAAVEAAGQARDELAGEAPSLAVLLGSRAHTDRAADVLSAVLQMIDPPALVGCIAQAIVAGRHEIEDEPAVVVWLASGLAAETFQLDFVRTGSGALITGYRFDRTARDLHLLLPDPYTFPSNLLIEHPNTDLPGTAVVGGVVSGGRRRGDTRLFRDHDVLTSGVVGVRLPGMRGVPVVSQGCRPIGYPYIVTGADGILITELGGRPPLQRLREIVEGLSPDERALVSHGLQIGIVVDEHLAAPGQGDFVIRGLLGADPSTGSIEIDEVVQVGATMQFQVRDAAGADKDLRLTVERAAARLPGRAAGALLFTCNGRGRRMFGVADHDASTIEELLGGIPLAGFFAAGEIGPIAGRNALHGFTASMALFVDDME