fprB Resolved · high auto-curated
H37Rv Rv0886 · MTBC0 mtbc0_000941 ·
575 aa · 987017–988744 (+) ·
RefSeq NP_215401.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | ferredoxin/ferredoxin--NADP reductase |
|---|---|
| MTBC0 PGAP re-annotation | FAD-dependent oxidoreductase |
| Revised (this work) | FAD-dependent oxidoreductase. Pfam: Fer4 (PF00037.33), Pyr_redox_2 (PF07992.21), NAD_binding_8 (PF13450.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJI1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable ferredoxin/ferredoxin--NADP reductase |
| EC (curated) |
EC 1.18.1.2
|
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversion
|
|---|---|
| Preferred name | fprB |
| eggNOG description | ferredoxin |
| Orthologous group | COG0493 |
| EC number |
EC 1.18.1.2, EC 1.19.1.1
|
| KEGG orthology |
K00528
|
| Gene Ontology (15) |
GO:0003674, GO:0003824, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005886, GO:0008150, GO:0008152, GO:0016020, GO:0016491, GO:0044424 +3 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.277 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Fer4 | PF00037.33 | 6.4e-06 | 40–61 | 4Fe-4S binding domain |
Pyr_redox_2 | PF07992.21 | 6.2e-08 | 114–272 | Pyridine nucleotide-disulphide oxidoreductase |
NAD_binding_8 | PF13450.13 | 5.9e-05 | 117–161 | NAD(P)-binding Rossmann-like domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: gltB (glutamate synthase large subunit), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3859c gltB |
glutamate synthase large subunit | 999 | 1000 ctx | neighborhood:544 coexpression:999 experimental:999 textmining:621 |
Rv1937 |
oxygenase | 995 | 990 | experimental:973 database:568 textmining:519 |
Rv0885 hyp |
hypothetical protein | 983 | 978 ctx | neighborhood:857 coexpression:798 |
Rv3106 fprA |
NADPH-ferredoxin reductase FprA | 945 | 943 | database:900 |
Rv2455c korA |
2-oxoglutarate oxidoreductase subunit KorA | 941 | 856 ctx | neighborhood:544 coexpression:698 textmining:611 |
Rv3554 fdxB |
electron transfer protein FdxB | 918 | 776 | experimental:430 database:568 textmining:651 |
Rv3230c |
stearoyl-CoA 9-desaturase electron transfer protein | 867 | 775 | experimental:430 database:568 textmining:436 |
Rv3571 kshB |
3-ketosteroid-9-alpha-hydroxylase reductase subunit | 866 | 773 | experimental:430 database:568 textmining:436 |
Rv2776c |
oxidoreductase | 866 | 773 | experimental:430 database:568 textmining:436 |
Rv1240 mdh |
malate dehydrogenase | 787 | 748 | coexpression:699 |
Rv0884c serC |
phosphoserine aminotransferase | 749 | 748 ctx | neighborhood:742 |
Rv0066c icd2 |
isocitrate dehydrogenase | 746 | 709 | coexpression:698 |
Rv0883c sepH hyp |
hypothetical protein | 669 | 670 ctx | neighborhood:670 |
Rv3338 |
Rv3338, (MTV016.38), len: 214 aa. Hypothetical protein, equivalent to C-termini of Q49926|ML0685 TPEA (putative hydrolase) from Mycobacteriu | 609 | 609 | coexpression:553 |
Rv1286 cysC |
adenylyl-sulfate kinase | 743 | 579 ctx | neighborhood:509 textmining:417 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: ferredoxin/ferredoxin--NADP reductase
- MTBC0 PGAP product: FAD-dependent oxidoreductase
- Pfam (hmmscan --cut_ga): Fer4 PF00037.33 (E=6e-06), Pyr_redox_2 PF07992.21 (E=6e-08), NAD_binding_8 PF13450.13 (E=6e-05)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215401.1)
- Domains: Pfam-A via hmmscan --cut_ga — Fer4 (PF00037.33), Pyr_redox_2 (PF07992.21), NAD_binding_8 (PF13450.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0493 - Curated reference: UniProt P9WJI1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
86 functional partner(s); context anchor
gltB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000941|Rv0886|fprB MPHVITQSCCNDASCVFACPVNCIHPTPDEPGFATSEMLYIDPVACVDCGACVTACPVSAIAPNTRLDFEQLPFVEINASYYPKRPAGVKLAPTSKLAPVTPAAEVRVRRQPLTVAVVGSGPAAMYAADELLVQQGVQVNVFEKLPTPYGLVRSGVAPDHQNTKRVTRLFDRIAGHRRFRFYLNVEIGKHLGHAELLAHHHAVLYAVGAPDDRRLTIDGMGLPGTGTATELVAWLNGHPDFNDLPVDLSHERVVIIGNGNVALDVARVLAADPHELAATDIADHALSALRNSAVREVVVAARRGPAHSAFTLPELIGLTAGADVVLDPGDHQRVLDDLAIVADPLTRNKLEILSTLGDGSAPARRVGRPRIRLAYRLTPRRVLGQRRAGGVQFSVTGTDELRQLDAGLVLTSIGYRGKPIPDLPFDEQAALVPNDGGRVIDPGTGEPVPGAYVAGWIKRGPTGFIGTNKSCSMQTVQALVADFNDGRLTDPVATPTALDQLVQARQPQAIGCAGWRAIDAAEIARGSADGRVRNKFTDVAEMLAAATSAPKEPLRRRVLARLRDLGQPIVLTVPL