pirG Resolved · medium auto-curated
H37Rv Rv3810 · MTBC0 mtbc0_004038 ·
284 aa · 4297849–4298703 (+) ·
RefSeq NP_218327.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cell surface protein |
|---|---|
| MTBC0 PGAP re-annotation | exported repetitive protein |
| Revised (this work) | Exported repetitive protein. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WIQ7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Exported repetitive protein |
| Curated function | Surface-exposed protein required for multiplication and intracellular growth. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| Preferred name | erp |
|---|---|
| Orthologous group | 2EIXY |
| Gene Ontology (12) |
GO:0005575, GO:0005576, GO:0008150, GO:0009405, GO:0040007, GO:0044110, GO:0044116, GO:0044117, GO:0044119, GO:0044403, GO:0044419, GO:0051704
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.171 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: eccD3 (ESX-3 secretion system protein EccD), high confidence from genomic context alone (score 769 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2721c hyp |
hypothetical protein | 918 | 918 ctx | cooccurence:709 coexpression:730 |
Rv0312 hyp |
hypothetical protein | 865 | 860 ctx | cooccurence:452 coexpression:754 |
Rv0129c fbpC |
diacylglycerol acyltransferase/mycolyltransferase Ag85C | 853 | 852 | coexpression:765 |
Rv1477 ripA |
peptidoglycan endopeptidase RipA | 843 | 843 | coexpression:801 |
Rv1158c hyp |
hypothetical protein | 831 | 824 | coexpression:772 |
Rv0040c mtc28 hyp |
hypothetical protein | 812 | 812 | coexpression:812 |
Rv1566c ripD hyp |
hypothetical protein | 809 | 802 | coexpression:802 |
Rv3811 hyp |
hypothetical protein | 787 | 779 ctx | neighborhood:714 |
Rv1157c hyp |
hypothetical protein | 775 | 774 | coexpression:733 |
Rv3707c hyp |
hypothetical protein | 770 | 770 ctx | cooccurence:769 |
Rv0290 eccD3 |
ESX-3 secretion system protein EccD | 769 | 769 ctx | cooccurence:766 |
Rv0479c |
membrane protein | 767 | 767 ctx | cooccurence:759 |
Rv3446c hyp |
hypothetical protein | 770 | 762 ctx | cooccurence:761 |
Rv2743c hyp |
hypothetical protein | 762 | 762 ctx | cooccurence:760 |
Rv0256c PPE2 |
PPE family protein PPE2 | 761 | 762 ctx | cooccurence:758 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: cell surface protein
- MTBC0 PGAP product: exported repetitive protein
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218327.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2EIXY - Curated reference: UniProt P9WIQ7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
162 functional partner(s); context anchor
eccD3 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_004038|Rv3810|pirG MPNRRRRKLSTAMSAVAALAVASPCAYFLVYESTETTERPEHHEFKQAAVLTDLPGELMSALSQGLSQFGINIPPVPSLTGSGDASTGLTGPGLTSPGLTSPGLTSPGLTDPALTSPGLTPTLPGSLAAPGTTLAPTPGVGANPALTNPALTSPTGATPGLTSPTGLDPALGGANEIPITTPVGLDPGADGTYPILGDPTLGTIPSSPATTSTGGGGLVNDVMQVANELGASQAIDLLKGVLMPSIMQAVQNGGAAAPAASPPVPPIPAAAAVPPTDPITVPVA