Rv3802c Resolved · high auto-curated
H37Rv Rv3802c · MTBC0 mtbc0_004030 ·
336 aa · 4287465–4288475 (-) ·
RefSeq NP_218319.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | membrane protein |
|---|---|
| MTBC0 PGAP re-annotation | phospholipase |
| Revised (this work) | Phospholipase. Pfam: Cutinase (PF01083.29), PE-PPE (PF08237.18). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O53581
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Carboxylesterase/lipase Culp6 |
| EC (curated) |
EC 3.1.1.-
|
| Curated function | Shows esterase and phospholipase A activities. May be involved in cell wall biosynthesis and/or maintenance. Can hydrolyze various substrates, including the p-nitrophenol-linked aliphatic esters pNP-laurate (C12), pNP-myristate (C14), pNP-palmitate (C16), pNP-stearate (C18), pNP-butyrate (C4), phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, 4-methylumbelliferyl heptanoate and palmitic acid and arachidonic acid containing phospholipids. Does not exhibit cutinase activity. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | elrF |
| eggNOG description | cutinase |
| Orthologous group | COG4223 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.848 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 5 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.26% of strains (384) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Cutinase | PF01083.29 | 5.3e-15 | 110–275 | Cutinase |
PE-PPE | PF08237.18 | 2.7e-07 | 120–310 | PE-PPE domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: fbpD (MPT51/MPB51 antigen), high confidence from genomic context alone (score 888 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3803c fbpD |
MPT51/MPB51 antigen | 891 | 888 ctx | neighborhood:737 cooccurence:589 |
Rv3800c pks13 |
polyketide synthase | 905 | 874 ctx | neighborhood:719 cooccurence:564 |
Rv3805c aftB |
terminal beta-(1->2)-arabinofuranosyltransferase | 852 | 848 ctx | cooccurence:771 |
Rv3804c fbpA |
diacylglycerol acyltransferase/mycolyltransferase Ag85A | 833 | 808 ctx | cooccurence:702 |
Rv3794 embA |
arabinosyltransferase A | 807 | 808 ctx | cooccurence:773 |
Rv3795 embB |
arabinosyltransferase B | 805 | 806 ctx | cooccurence:771 |
Rv3793 embC |
arabinosyltransferase C | 805 | 805 ctx | cooccurence:771 |
Rv3801c fadD32 |
long-chain-fatty-acid--AMP ligase FadD32 | 838 | 779 ctx | neighborhood:719 |
Rv3668c |
protease | 778 | 779 ctx | cooccurence:769 |
Rv0129c fbpC |
diacylglycerol acyltransferase/mycolyltransferase Ag85C | 800 | 778 ctx | cooccurence:743 |
Rv3244c lpqB |
lipoprotein LpqB | 774 | 774 ctx | cooccurence:764 |
Rv2673 aftC |
alpha-(1->3)-arabinofuranosyltransferase | 774 | 774 ctx | cooccurence:774 |
Rv1476 |
membrane protein | 774 | 774 ctx | cooccurence:773 |
Rv3035 hyp |
hypothetical protein | 770 | 771 ctx | cooccurence:767 |
Rv2342 hyp |
hypothetical protein | 761 | 762 ctx | cooccurence:756 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: membrane protein
- MTBC0 PGAP product: phospholipase
- Pfam (hmmscan --cut_ga): Cutinase PF01083.29 (E=5e-15), PE-PPE PF08237.18 (E=3e-07)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218319.1)
- Domains: Pfam-A via hmmscan --cut_ga — Cutinase (PF01083.29), PE-PPE (PF08237.18)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4223 - Curated reference: UniProt O53581 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
128 functional partner(s); context anchor
fbpD - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_004030|Rv3802c| MAKNSRRKRHRILAWIAAGAMASVVALVIVAVVIMLRGAESPPSAVPPGVLPPGPTPAHPHKPRPAFQDASCPDVQMISVPGTWESSPQQNPLNPVQFPKALLLKVTGPIAQQFAPARVQTYTVAYTAQFHNPLTTDNQMSYNDSRAEGTRAMVAAMTDMNNRCPLTSYVLIGFSQGAVIAGDVASDIGNGRGPVDEDLVLGVTLIADGRRQQGVGNQVPPSPRGEGAEITLHEVPVLSGLGLTMTGPRPGGFGALDGRTNEICAQGDLICAAPAQAFSPANLPTTLNTLAGGAGQPVHAMYATPEFWNSDGEPATEWTLNWAHQLIENAPHPKHR