Rv3740c Family assigned · medium auto-curated
H37Rv Rv3740c · MTBC0 mtbc0_003964 ·
448 aa · 4214954–4216300 (-) ·
RefSeq NP_218257.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | diacyglycerol O-acyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | wax ester/triacylglycerol synthase family O-acyltransferase |
| Revised (this work) | Wax ester/triacylglycerol synthase family O-acyltransferase. Pfam: WS_DGAT_cat (PF03007.22), WS_DGAT_C (PF06974.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WKA5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative diacyglycerol O-acyltransferase Rv3740c |
| EC (curated) |
EC 2.3.1.20
|
| Curated function | Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of palmitoyl-CoA. |
UniProt still lists this protein as Putative diacyglycerol O-acyltransferase Rv3740c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Wax ester synthase-like Acyl-CoA acyltransferase domain |
| Orthologous group | COG1020 |
| Gene Ontology (64) |
GO:0000302, GO:0001666, GO:0003674, GO:0003824, GO:0004144, GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0006629, GO:0006638, GO:0006639 +52 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.273 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 6 missense, 1 nonsense, 4 frameshift |
| Disruption | 5 distinct premature-stop/frameshift site(s); most common in 0.64% of strains (931) · convergent |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
WS_DGAT_cat | PF03007.22 | 1.4e-98 | 1–257 | Wax ester synthase/diacylglycerol acyltransferase catalytic domain |
WS_DGAT_C | PF06974.19 | 1.8e-40 | 297–442 | WS/DGAT C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3741c (oxidoreductase), high confidence from genomic context alone (score 981 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3741c |
oxidoreductase | 980 | 981 ctx | neighborhood:882 coexpression:822 |
Rv3742c |
oxidoreductase | 978 | 979 ctx | neighborhood:882 coexpression:804 |
Rv3097c lipY |
triacylglycerol lipase Lip | 919 | 919 | database:900 |
Rv2252 dagK |
diacylglycerol kinase | 900 | 901 | database:900 |
Rv3391 acrA1 |
acyl-CoA-reductase AcrA | 771 | 762 ctx | cooccurence:745 |
Rv1543 |
oxidoreductase | 728 | 729 ctx | cooccurence:722 |
Rv0547c |
oxidoreductase | 669 | 669 ctx | cooccurence:661 |
Rv2627c hyp |
hypothetical protein | 664 | 652 ctx | cooccurence:436 |
Rv0825c hyp |
hypothetical protein | 600 | 600 ctx | cooccurence:594 |
Rv3825c pks2 |
phthioceranic/hydroxyphthioceranic acid synthase | 533 | 506 | experimental:441 |
Rv2048c pks12 |
polyketide synthase | 527 | 500 | experimental:441 |
Rv2933 ppsC |
phthiocerol synthesis polyketide synthase type I PpsC | 527 | 500 | experimental:441 |
Rv2940c mas |
multifunctional mycocerosic acid synthase | 527 | 500 | experimental:441 |
Rv3743c ctpJ |
cation transporter ATPase J | 500 | 500 ctx | neighborhood:497 |
Rv1527c pks5 |
polyketide synthase | 526 | 499 | experimental:441 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: diacyglycerol O-acyltransferase
- MTBC0 PGAP product: wax ester/triacylglycerol synthase family O-acyltransferase
- Pfam (hmmscan --cut_ga): WS_DGAT_cat PF03007.22 (E=1e-98), WS_DGAT_C PF06974.19 (E=2e-40)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218257.1)
- Domains: Pfam-A via hmmscan --cut_ga — WS_DGAT_cat (PF03007.22), WS_DGAT_C (PF06974.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1020 - Curated reference: UniProt P9WKA5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
27 functional partner(s); context anchor
Rv3741c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003964|Rv3740c| MSPIDALFLSAESREHPLHVGALQLFEPPAGAGRGFVRETYQAMLQCREIAPLFRKRPTSLHGALINLGWSTDADVDLGYHARRSALPAPGRVRELLELTSRLHSNLLDRHRPLWETHVIEGLRDGRFAIYSKMHHALVDGVSGLTLMRQPMTTDPIEGKLRTAWSPATQHTAIKRRRGRLQQLGGMLGSVAGLAPSTLRLARSALIEQQLTLPFGAPHTMLNVAVGGARRCAAQSWPLDRVKAVKDAAGVSLNDVVLAMCAGALREYLDDNDALPDTPLVAMVPVSLRTDRDSVGGNMVGAVLCNLATHLDDPADRLNAIHASMRGNKNVLSQLPRAQALAVSLLLLSPAALNTLPGLAKATPPPFNVCISNVPGAREPLYFNGARMVGNYPMSLVLDGQALNITLTSTADSLDFGVVGCRRSVPHVQRVLSHLETSLKELERAVGL