Rv0547c Family assigned · medium auto-curated
H37Rv Rv0547c · MTBC0 mtbc0_000576 ·
294 aa · 641583–642467 (-) ·
RefSeq NP_215061.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | oxidoreductase |
|---|---|
| MTBC0 PGAP re-annotation | SDR family oxidoreductase |
| Revised (this work) | SDR family oxidoreductase. Pfam: adh_short (PF00106.32), adh_short_C2 (PF13561.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06413
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Fatty acyl-CoA reductase Rv0547c |
| EC (curated) |
EC 1.2.1.-
|
| Curated function | Oxidoreductase that promotes the persistence of M.tuberculosis in host macrophages by reprogramming the fatty acid metabolism in host mitochondria. When localized in the host mitochondria, it potentially acts on unknown lipid substrates and converts them into products that directly or indirectly alter the lipid profile of the mitochondria. This change in lipid profile results in increased mitochondrial membrane fluidity, enhanced endogenous fatty acid oxidation and increased mitochondrial spare respiratory capacity. All these events eventually favor M.tuberculosis persistence in the host macro. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Belongs to the short-chain dehydrogenases reductases (SDR) family |
| Orthologous group | COG0300 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.724 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
adh_short | PF00106.32 | 1.1e-37 | 41–233 | short chain dehydrogenase |
adh_short_C2 | PF13561.13 | 1.4e-28 | 47–241 | Enoyl-(Acyl carrier protein) reductase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0544c (transmembrane protein), high confidence from genomic context alone (score 716 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0544c |
transmembrane protein | 715 | 716 ctx | neighborhood:714 |
Rv0545c pitA |
low-affinity inorganic phosphate transporter | 715 | 716 ctx | neighborhood:714 |
Rv3485c |
short-chain type dehydrogenase/reductase | 704 | 693 ctx | cooccurence:651 |
Rv3740c |
diacyglycerol O-acyltransferase | 669 | 669 ctx | cooccurence:661 |
Rv3734c tgs2 |
diacyglycerol O-acyltransferase | 666 | 667 ctx | cooccurence:657 |
Rv0548c menB |
1,4-dihydroxy-2-naphthoyl-CoA synthase | 662 | 662 ctx | neighborhood:657 |
Rv3480c |
diacyglycerol O-acyltransferase | 658 | 659 ctx | cooccurence:640 |
Rv0542c menE |
2-succinylbenzoic acid--CoA ligase | 662 | 653 ctx | neighborhood:651 |
Rv0541c |
integral membrane protein | 639 | 638 ctx | neighborhood:635 |
Rv0551c fadD8 |
fatty-acid--CoA ligase FadD8 | 641 | 632 ctx | neighborhood:630 |
Rv2285 |
diacylglycerol acyltransferase | 631 | 632 ctx | cooccurence:614 |
Rv0895 |
diacyglycerol O-acyltransferase | 627 | 627 ctx | cooccurence:617 |
Rv1714 |
oxidoreductase | 624 | 615 ctx | cooccurence:560 |
Rv0546c hyp |
hypothetical protein | 597 | 597 ctx | neighborhood:590 |
Rv3088 tgs4 |
diacyglycerol O-acyltransferase | 571 | 572 ctx | cooccurence:569 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: oxidoreductase
- MTBC0 PGAP product: SDR family oxidoreductase
- Pfam (hmmscan --cut_ga): adh_short PF00106.32 (E=1e-37), adh_short_C2 PF13561.13 (E=1e-28)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215061.1)
- Domains: Pfam-A via hmmscan --cut_ga — adh_short (PF00106.32), adh_short_C2 (PF13561.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0300 - Curated reference: UniProt O06413 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
38 functional partner(s); context anchor
Rv0544c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000576|Rv0547c| MSKRPLRWLTEQITLAGMRPPISPQLLINRPAMQPVDLTGKRILLTGASSGIGAAATKQFGLHRAVVVAVARRKDLLDAVADRITGDGGTAMSLPCDLSDMEAIDALVEDVEKRIGGIDILINNAGRSIRRPLAESLERWHDVERTMVLNYYAPLRLIRGLAPGMLERGDGHIINVATWGVLSEASPLFSVYNASKAALSAVSRIIETEWGSQGVHSTTLYYPLVATPMIAPTKAYDGLPALTAAEAAEWMVTAARTRPVRIAPRVAVAVNALDSIGPRWVNALMQRRNEQLNP