Rv3737 Family assigned · medium auto-curated
H37Rv Rv3737 · MTBC0 mtbc0_003962 ·
529 aa · 4211820–4213409 (+) ·
RefSeq NP_218254.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane protein |
|---|---|
| MTBC0 PGAP re-annotation | threonine/serine exporter family protein |
| Revised (this work) | Threonine/serine exporter family protein. Pfam: ThrE (PF06738.19), ThrE_2 (PF12821.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O69704
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable threonine/serine exporter |
| Curated function | Catalyzes the export of L-threonine and L-serine from the cell to the extracellular environment (By similarity). Export is dependent on the proton motive force (By similarity). Required for in vitro growth and survival of bacteria inside macrophages. Increased expression is associated with low-level amikacin (AMK) resistance. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Threonine/Serine exporter, ThrE |
| Orthologous group | COG2966 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.572 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 7 synonymous, 9 missense, 1 nonsense, 1 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.14% of strains (202) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ThrE | PF06738.19 | 1.3e-63 | 50–287 | Putative threonine/serine exporter |
ThrE_2 | PF12821.14 | 8.2e-11 | 323–446 | Threonine/Serine exporter, ThrE |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3736 (AraC/XylS family transcriptional regulator), high confidence from genomic context alone (score 805 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3736 |
AraC/XylS family transcriptional regulator | 973 | 805 ctx | neighborhood:801 textmining:870 |
Rv3882c eccE1 |
ESX-1 secretion system protein EccE1 | 668 | 668 ctx | cooccurence:668 |
Rv3735 hyp |
hypothetical protein | 647 | 647 ctx | neighborhood:646 |
Rv3877 eccD1 |
ESX-1 secretion system protein EccD1 | 602 | 603 ctx | cooccurence:600 |
Rv1407 fmu |
16S rRNA m5C967 methyltransferase | 568 | 568 ctx | neighborhood:544 |
Rv1832 gcvB |
glycine dehydrogenase | 567 | 567 ctx | neighborhood:544 |
Rv0014c pknB |
serine/threonine-protein kinase PknB | 550 | 550 ctx | neighborhood:544 |
Rv0931c pknD |
serine/threonine-protein kinase PknD | 549 | 550 ctx | neighborhood:544 |
Rv0901 arfC |
membrane protein | 546 | 547 ctx | cooccurence:544 |
Rv2006 otsB1 |
trehalose-6-phosphate phosphatase OtsB | 545 | 545 ctx | neighborhood:544 |
Rv0658c |
integral membrane protein | 544 | 544 ctx | cooccurence:541 |
Rv2416c eis |
enhanced intracellular survival protein | 540 | 541 ctx | cooccurence:475 |
Rv2124c metH |
methionine synthase | 539 | 539 ctx | neighborhood:539 |
Rv1226c |
transmembrane protein | 482 | 482 ctx | cooccurence:463 |
Rv3875 esxA |
ESAT-6 protein EsxA | 469 | 470 ctx | cooccurence:467 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane protein
- MTBC0 PGAP product: threonine/serine exporter family protein
- Pfam (hmmscan --cut_ga): ThrE PF06738.19 (E=1e-63), ThrE_2 PF12821.14 (E=8e-11)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218254.1)
- Domains: Pfam-A via hmmscan --cut_ga — ThrE (PF06738.19), ThrE_2 (PF12821.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2966 - Curated reference: UniProt O69704 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
33 functional partner(s); context anchor
Rv3736 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003962|Rv3737| MDQDRSDNTALRRGLRIALRGRRDPLPVAGRRSRTSGGIGDLHTRKVLDLTIRLAEVMLSSGSGTADVVATAQDVAQAYQLTDCVVDITVTTIIVSALATTDTPPVTIMRSVRTRSTDYSRLAELDRLVQRITSGGVAVDQAHEAMDELTERPHPYPRWLATAGAAGFALGVAMLLGGTWLTCVLAAVTSGVIDRLGRLLNRIGTPLFFQRVFGAGIATLVAVAAYLIAGQDPTALVATGIVVLLSGMTLVGSMQDAVTGYMLTALARLGDALFLTAGIVVGILISLRGVTNAGIQIELHVDATTTLATPGMPLPILVAVSGAALSGVCLTIASYAPLRSVATAGLSAGLAELVLIGLGAAGFGRVVATWTAAIGVGFLATLISIRRQAPALVTATAGIMPMLPGLAVFRAVFAFAVNDTPDGGLTQLLEAAATALALGSGVVLGEFLASPLRYGAGRIGDLFRIEGPPGLRRAVGRVVRLQPAKSQQPTGTGGQRWRSVALEPTTADDVDAGYRGDWPATCTSATEVR