Rv3638 Family assigned · medium auto-curated
H37Rv Rv3638 · MTBC0 mtbc0_003854 ·
132 aa · 4100688–4101435 (+) ·
RefSeq NP_218155.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transposase |
|---|---|
| MTBC0 PGAP re-annotation | ATP-binding protein |
| Revised (this work) | ATP-binding protein. Pfam: IstB_IS21 (PF01695.24). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6XHU7
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Possible transposase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
L Replication, recombination and repair
|
|---|---|
| eggNOG description | IstB-like ATP binding protein |
| Orthologous group | COG1484 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.718 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 4 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.26% of strains (377) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
IstB_IS21 | PF01695.24 | 1.7e-14 | 21–107 | IstB-like ATP binding protein |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3637 (Possible transposase; Rv3637, (MTCY15C10.15c), len: 166 aa. Possible transposase. C-terminal end highly similar to Q9RLQ9|ISTA putative tran), high confidence from genomic context alone (score 980 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3637 |
Possible transposase; Rv3637, (MTCY15C10.15c), len: 166 aa. Possible transposase. C-terminal end highly similar to Q9RLQ9|ISTA putative tran | 980 | 980 ctx | neighborhood:882 cooccurence:729 coexpression:412 |
Rv3428c |
transposase | 833 | 827 ctx | cooccurence:771 |
Rv0058 dnaB |
replicative DNA helicase | 814 | 797 | experimental:773 |
Rv2943 |
insertion sequence element IS1533 transposase | 804 | 797 ctx | cooccurence:773 |
Rv2813 hyp |
hypothetical protein | 774 | 775 | coexpression:772 |
Rv3636 |
Possible transposase; Rv3636, (MTCY15C10.16c), len: 115 aa. Possible transposase, weakly similar to others e.g. O69924|SC3C8.12 putative tra | 738 | 728 ctx | neighborhood:419 cooccurence:526 |
Rv0943c |
monooxygenase | 474 | 474 | coexpression:474 |
Rv1051c hyp |
hypothetical protein | 450 | 451 | coexpression:441 |
Rv0071 |
maturase | 413 | 408 ctx | cooccurence:400 |
Rv0620 galK |
galactokinase | 413 | 392 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transposase
- MTBC0 PGAP product: ATP-binding protein
- Pfam (hmmscan --cut_ga): IstB_IS21 PF01695.24 (E=2e-14)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218155.1)
- Domains: Pfam-A via hmmscan --cut_ga — IstB_IS21 (PF01695.24)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1484 - Curated reference: UniProt I6XHU7 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
10 functional partner(s); context anchor
Rv3637 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003854|Rv3638| MAAKTATNSRDVAAELAYLTRALKAPTLRGAIEQLADRARTKTWSYEEFLAACLQREVSARESHGGEGRIRAARFPSRKSLEEFDFDHARGLKRDTIAHLGTLDFVTPSNRDRDPRLPGRPPRPIRHRLAMG