sseA Resolved · high auto-curated
H37Rv Rv3283 · MTBC0 mtbc0_003491 ·
297 aa · 3687008–3687901 (+) ·
RefSeq NP_217800.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | thiosulfate sulfurtransferase SseA |
|---|---|
| MTBC0 PGAP re-annotation | sulfurtransferase |
| Revised (this work) | Sulfurtransferase. Pfam: Rhodanese (PF00581.26). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WHF7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative thiosulfate sulfurtransferase SseA |
| EC (curated) |
EC 2.8.1.1
|
UniProt still lists this protein as Putative thiosulfate sulfurtransferase SseA; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | sseA |
| eggNOG description | sulfurtransferase |
| Orthologous group | COG2897 |
| EC number |
EC 2.8.1.1, EC 2.8.1.2
|
| KEGG orthology |
K01011
|
| KEGG pathways |
map00270, map00920, map01100, map01120, map04122
|
| Gene Ontology (13) |
GO:0005575, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0016020, GO:0030312, GO:0044424, GO:0044444, GO:0044464 +1 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.531 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 4 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.18% of strains (265) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Rhodanese | PF00581.26 | 2.3e-17 | 164–279 | Rhodanese-like domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: accD5 (propionyl-CoA carboxylase subunit beta), high confidence from genomic context alone (score 826 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3284 hyp |
hypothetical protein | 957 | 957 ctx | neighborhood:882 cooccurence:632 |
Rv2291 sseB |
thiosulfate sulfurtransferase SseB | 941 | 928 | database:900 |
Rv3025c iscS |
cysteine desulfurase | 922 | 915 | database:900 |
Rv0815c cysA2 |
thiosulfate sulfurtransferase CysA | 908 | 906 | database:900 |
Rv2391 sirA |
sulfite reductase | 912 | 904 | database:900 |
Rv2392 cysH |
phosphoadenosine phosphosulfate reductase | 909 | 904 | database:900 |
Rv0075 |
aminotransferase | 900 | 901 | database:900 |
Rv2294 |
cystathionine beta-lyase | 900 | 900 | database:900 |
Rv3280 accD5 |
propionyl-CoA carboxylase subunit beta | 877 | 826 ctx | neighborhood:822 |
Rv3282 hyp |
hypothetical protein | 830 | 823 ctx | neighborhood:822 |
Rv3281 accE5 |
bifunctional protein acetyl-/propionyl-CoAcarboxylase subunit epsilon AccE | 823 | 823 ctx | neighborhood:822 |
Rv3684 |
lyase | 851 | 820 | database:800 |
Rv2334 cysK1 |
O-acetylserine sulfhydrylase | 829 | 820 | database:800 |
Rv1079 metB |
cystathionine gamma-synthase | 839 | 816 | database:800 |
Rv0391 metZ |
O-succinylhomoserine sulfhydrylase | 830 | 816 | database:800 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: thiosulfate sulfurtransferase SseA
- MTBC0 PGAP product: sulfurtransferase
- Pfam (hmmscan --cut_ga): Rhodanese PF00581.26 (E=2e-17)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217800.1)
- Domains: Pfam-A via hmmscan --cut_ga — Rhodanese (PF00581.26)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2897 - Curated reference: UniProt P9WHF7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
33 functional partner(s); context anchor
accD5 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003491|Rv3283|sseA MPLPADPSPTLSAYAHPERLVTADWLSAHMGAPGLAIVESDEDVLLYDVGHIPGAVKIDWHTDLNDPRVRDYINGEQFAELMDRKGIARDDTVVIYGDKSNWWAAYALWVFTLFGHADVRLLNGGRDLWLAERRETTLDVPTKTCTGYPVVQRNDAPIRAFRDDVLAILGAQPLIDVRSPEEYTGKRTHMPDYPEEGALRAGHIPTAVHIPWGKAADESGRFRSREELERLYDFINPDDQTVVYCRIGERSSHTWFVLTHLLGKADVRNYDGSWTEWGNAVRVPIVAGEEPGVVPVV