Rv2844 Family assigned · low auto-curated
H37Rv Rv2844 · MTBC0 mtbc0_003023 ·
162 aa · 3171379–3171867 (+) ·
RefSeq NP_217360.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | ferritin-like domain-containing protein |
| Revised (this work) | Ferritin-like domain-containing protein. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6Y1V1
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Conserved alanine rich protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Domain of unknown function (DUF4439) |
| Orthologous group | 2EA6A |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DUF4439 | PF14530.13 | 7.4e-46 | 25–161 | Domain of unknown function (DUF4439) |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: nusA (transcription termination/antitermination protein NusA), high confidence from genomic context alone (score 762 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2843 hyp |
hypothetical protein | 913 | 913 ctx | neighborhood:882 |
Rv2841c nusA |
transcription termination/antitermination protein NusA | 762 | 762 ctx | neighborhood:760 |
Rv2842c rimP |
ribosome maturation factor RimP | 761 | 762 ctx | neighborhood:760 |
Rv3118 sseC1 hyp |
hypothetical protein | 727 | 728 ctx | cooccurence:726 |
Rv0814c sseC2 hyp |
hypothetical protein | 726 | 727 ctx | cooccurence:726 |
Rv3605c hyp |
hypothetical protein | 660 | 660 ctx | cooccurence:660 |
Rv3231c hyp |
hypothetical protein | 623 | 624 ctx | cooccurence:615 |
Rv2728c hyp |
hypothetical protein | 619 | 619 ctx | cooccurence:618 |
Rv1332 |
transcriptional regulator | 609 | 609 ctx | cooccurence:608 |
Rv2468c hyp |
hypothetical protein | 598 | 598 ctx | cooccurence:589 |
Rv0010c |
membrane protein | 569 | 569 ctx | cooccurence:568 |
Rv3839 hyp |
hypothetical protein | 561 | 561 ctx | cooccurence:559 |
Rv1486c hyp |
hypothetical protein | 551 | 551 ctx | cooccurence:550 |
Rv1473A |
transcriptional regulator | 546 | 546 ctx | cooccurence:541 |
Rv2712c hyp |
hypothetical protein | 538 | 538 ctx | cooccurence:537 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: ferritin-like domain-containing protein
- Pfam (hmmscan --cut_ga): DUF4439 PF14530.13 (E=7e-46)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217360.1)
- Domains: Pfam-A via hmmscan --cut_ga — DUF4439 (PF14530.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2EA6A - Curated reference: UniProt I6Y1V1 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
35 functional partner(s); context anchor
nusA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003023|Rv2844| MTSSEPAHGATPKRSPSEGSADNAALCDALAVEHATIYGYGIVSALSPPGVNFLVADALKQHRHRRDDVIVMLSARGVTAPIAAAGYQLPMQVSSAADAARLAVRMENDGATAWRAVVEHAETADDRVFASTALTESAVMATRWNRVLGAWPITAAFPGGDE