Rv2611c Resolved · high auto-curated
H37Rv Rv2611c · MTBC0 mtbc0_002779 ·
316 aa · 2962565–2963515 (-) ·
RefSeq NP_217127.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | phosphatidylinositol mannoside acyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | phosphatidylinositol mannoside acyltransferase |
| Revised (this work) | Phosphatidylinositol mannoside acyltransferase. Pfam: Lip_A_acyltrans (PF03279.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WMB5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Phosphatidylinositol mannoside acyltransferase |
| EC (curated) |
EC 2.3.1.265
|
| Curated function | Catalyzes the transfer of a palmitoyl moiety from palmitoyl-CoA to the 6-position of the mannose ring linked to the 2-position of myo-inositol in phosphatidyl-myo-inositol monomannoside (PIM1) or dimannoside (PIM2). Essential for growth and survival in axenic cultures and during macrophage infection and in a mouse model of infection. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| Preferred name | htrB |
| eggNOG description | Acyltransferase |
| Orthologous group | COG1560 |
| EC number |
EC 2.3.1.265
|
| KEGG orthology |
K22311
|
| Gene Ontology (26) |
GO:0003674, GO:0003824, GO:0005575, GO:0006629, GO:0006643, GO:0006664, GO:0008150, GO:0008152, GO:0008610, GO:0009058, GO:0009247, GO:0009987 +14 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.224 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Lip_A_acyltrans | PF03279.19 | 1.4e-100 | 12–302 | Bacterial lipid A biosynthesis acyltransferase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pimA (alpha-(1-2)-phosphatidylinositol mannosyltransferase), high confidence from genomic context alone (score 996 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2610c pimA |
alpha-(1-2)-phosphatidylinositol mannosyltransferase | 999 | 996 ctx | neighborhood:879 cooccurence:725 database:900 textmining:897 |
Rv2612c pgsA1 |
CDP-diacylglycerol--inositol 3-phosphatidyltransferase | 992 | 969 ctx | neighborhood:882 fusion:463 cooccurence:448 textmining:753 |
Rv2188c pimB |
alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase | 997 | 960 ctx | cooccurence:594 database:900 textmining:928 |
Rv2613c |
AP-4-A phosphorylase | 964 | 906 ctx | neighborhood:882 textmining:638 |
Rv2609c |
membrane protein | 951 | 893 ctx | neighborhood:798 cooccurence:490 textmining:566 |
Rv2614c thrS |
threonine--tRNA ligase | 920 | 881 ctx | neighborhood:881 |
Rv2614A hyp |
hypothetical protein | 550 | 550 ctx | neighborhood:550 |
Rv0037c |
MFS-type transporter | 504 | 482 ctx | cooccurence:482 |
Rv3670 ephE |
epoxide hydrolase EphE | 452 | 453 ctx | cooccurence:445 |
Rv3259 hyp |
hypothetical protein | 449 | 449 ctx | cooccurence:447 |
Rv2965c kdtB |
phosphopantetheine adenylyltransferase | 442 | 442 | coexpression:418 |
Rv3231c hyp |
hypothetical protein | 421 | 421 ctx | cooccurence:421 |
Rv2588c yajC |
membrane protein secretion factor YajC | 424 | 371 | |
Rv2673 aftC |
alpha-(1->3)-arabinofuranosyltransferase | 435 | 316 | |
Rv3805c aftB |
terminal beta-(1->2)-arabinofuranosyltransferase | 641 | 192 | textmining:575 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: phosphatidylinositol mannoside acyltransferase
- MTBC0 PGAP product: phosphatidylinositol mannoside acyltransferase
- Pfam (hmmscan --cut_ga): Lip_A_acyltrans PF03279.19 (E=1e-100)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217127.1)
- Domains: Pfam-A via hmmscan --cut_ga — Lip_A_acyltrans (PF03279.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1560 - Curated reference: UniProt P9WMB5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
27 functional partner(s); context anchor
pimA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002779|Rv2611c| MIAGLKGLKLPKDPRSSVTRTATDWAYAAGWMAVRALPEFAVRNAFDTGARYFARHGGPEQLRKNLARVLGVPPAAVPDPLMCASLESYGRYWREVFRLPTMNHRKLARQLDRVIGGLDHLDAALAAGLGAVLALPHSGNWDMAGMWLVQRHGTFTTVAERLKPESLYQRFIDYRESLGFEVLPLSGGERPPFEVLCERLRNNRVVCLMAERDLTRTGVEVDFFGEPTRMPVGPAKLAVETGAALLPTHCWFEGRGWGFQVYPALDCTSGDVAAITQALADRFAQNIAAHPADWHMLQPQWLADLSESRRAQLRSR