mazF8 Resolved · medium auto-curated
H37Rv Rv2274c · MTBC0 mtbc0_002416 ·
105 aa · 2572593–2572910 (-) ·
RefSeq NP_216790.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | toxin MazF8 |
|---|---|
| MTBC0 PGAP re-annotation | toxin |
| Revised (this work) | Toxin. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WIH7
SwissProt · reviewed
· Uncertain
|
|---|---|
| UniProt name | Putative toxin MazF8 |
| EC (curated) |
EC 3.1.-.-
|
| Curated function | Putative toxic component of a type II toxin-antitoxin (TA) system, its cognate toxin is MaZE8. Probably an endoribonuclease (By similarity). |
UniProt still lists this protein as Putative toxin MazF8; the revised annotation above is ahead of the current UniProt record.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.112 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mazE8 (antitoxin MazE8), medium confidence from genomic context alone (score 668 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2274A mazE8 |
antitoxin MazE8 | 879 | 668 ctx | neighborhood:668 textmining:653 |
Rv2275 |
cyclo(L-tyrosyl-L-tyrosyl) synthase | 570 | 570 ctx | neighborhood:556 |
Rv2276 cyp121 |
cytochrome P450 Cyp121 | 532 | 532 ctx | neighborhood:525 |
Rv0656c vapC6 |
ribonuclease VapC6 | 575 | 51 | textmining:571 |
Rv1720c vapC12 |
ribonuclease VapC12 | 523 | 47 | textmining:520 |
Rv1397c vapC10 |
ribonuclease VapC10 | 520 | 44 | textmining:519 |
Rv3905c esxF |
ESAT-6 like protein EsxF | 437 | 44 | textmining:436 |
Rv2138 lppL |
lipoprotein LppL | 423 | 44 | textmining:422 |
Rv1004c |
membrane protein | 629 | 43 | textmining:629 |
Rv2760c vapB42 |
antitoxin VapB42 | 440 | 42 | textmining:440 |
Rv3289c |
transmembrane protein | 629 | 41 | textmining:629 |
Rv0996 |
transmembrane protein | 546 | 41 | textmining:546 |
Rv2526 vapB17 |
antitoxin VapB17 | 511 | 41 | textmining:511 |
Rv3408 vapC47 |
ribonuclease VapC47 | 510 | 41 | textmining:510 |
Rv2615c PE_PGRS45 |
PE-PGRS family protein PE_PGRS45 | 437 | 41 | textmining:437 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: toxin MazF8
- MTBC0 PGAP product: toxin
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216790.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Curated reference: UniProt P9WIH7 (SwissProt, reviewed; Uncertain)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
16 functional partner(s); context anchor
mazE8 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002416|Rv2274c|mazF8 MSIARSAQPIGWISCPPKGGSSCCRCGGGYTHIFCVSAWTGLVVDLQAEQVRSVVTERLRRRIGRGAPILAGTLAPGVGLAAQNREFRQFTGRSAPPSATIAFGE