Rv1635c Family assigned · low auto-curated
H37Rv Rv1635c · MTBC0 mtbc0_001743 ·
556 aa · 1852536–1854206 (-) ·
RefSeq NP_216151.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | mannosyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | glycosyltransferase family 39 protein |
| Revised (this work) | Glycosyltransferase family 39 protein. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06152
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Probable mannosyltransferase. Probable conserved transmembrane protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| eggNOG description | Dolichyl-phosphate-mannose-protein mannosyltransferase |
| Orthologous group | COG1807 |
| KEGG orthology |
K14340
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.286 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0048c (membrane protein), high confidence from genomic context alone (score 713 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2079 hyp |
hypothetical protein | 729 | 730 ctx | cooccurence:729 |
Rv0048c |
membrane protein | 713 | 713 ctx | cooccurence:713 |
Rv1171 hyp |
hypothetical protein | 698 | 699 ctx | cooccurence:698 |
Rv0875c hyp |
hypothetical protein | 654 | 654 ctx | cooccurence:649 |
Rv3829c |
dehydrogenase | 623 | 623 ctx | cooccurence:623 |
Rv1636 TB15.3 |
iron-regulated universal stress protein | 618 | 619 ctx | neighborhood:617 |
Rv0188 |
transmembrane protein | 600 | 600 ctx | cooccurence:596 |
Rv2423 hyp |
hypothetical protein | 589 | 589 ctx | cooccurence:589 |
Rv3899c hyp |
hypothetical protein | 558 | 558 ctx | cooccurence:556 |
Rv3481c |
integral membrane protein | 545 | 545 ctx | cooccurence:545 |
Rv0648 |
alpha-mannosidase | 544 | 544 ctx | cooccurence:544 |
Rv3202c adnA |
ATP-dependent DNA helicase | 523 | 523 ctx | cooccurence:504 |
Rv1359 |
transcriptional regulator | 521 | 522 ctx | cooccurence:520 |
Rv3434c |
transmembrane protein | 517 | 518 ctx | cooccurence:516 |
Rv1353c |
HTH-type transcriptional regulator | 512 | 512 ctx | cooccurence:512 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: mannosyltransferase
- MTBC0 PGAP product: glycosyltransferase family 39 protein
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216151.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1807 - Curated reference: UniProt O06152 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
48 functional partner(s); context anchor
Rv0048c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001743|Rv1635c| MHASRPGAPPHAGLPSRRTAGDQDHRADPKVTRIMSASTLEQPAAAHVDELVARMRGRLLDPLAIAVLAAVISGAWASRPSLWFDEGATISASASRTLPELWSLLGHIDAVHGLYYLLMHGWFAIFPPTELWSRLPSCLAIGAAAAGVVVFAKQFSGRTTAVCAGAVFAILPRVTWAGIEARSSALSVAAAVWLTVLLVAAVRCNTQRRWLLYALVLMLSILVSINLALLVPAYATMVPLLASGKSRKSPVIWWTVVTAAALGAMTPFILFAHGQVWQVGWIAGLNRNIILDVIHRQYFDHSVPFAILAGLIVAAGIAAHLAGARGPGGDTHRLVLVSAAWIVVPTAVVLIYSATVEPIYYPRYLILTAPAAAVILAVCVVTIARKPWLIAGVVFLLAAAAFPNYFFTQRGPYAKEGWDYSQVADVISAHAKPGDCLLVDNTAGWRPGPIRALLATRPAAFRSLIDVERGTYGPKVGTLWDGHVAVWLTTAKIDKCPTLWTIANRDKSLPDHQVGEMLSPGTGFGRTPVYRFPSYLGFRIVERWQFHYSQVVKSTR