Rv0799c Resolved · high auto-curated
H37Rv Rv0799c · MTBC0 mtbc0_000848 ·
335 aa · 895333–896340 (-) ·
RefSeq NP_215314.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | Dyp-type peroxidase |
| Revised (this work) | Dyp-type peroxidase. Pfam: Dyp_perox_N (PF04261.19), Dyp_perox_C (PF20628.4). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6Y4U9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Dye-decolorizing peroxidase |
| EC (curated) |
EC 1.11.1.7
|
| Curated function | Cargo of a type 1 encapsulin nanocompartment in situ; this cargo protects against oxidative stress at low pH. When expressed in the cytoplasm (absence of the encapsulin shell gene) it is almost as protective as the intact nanocompartment; its encapsulation has a modest yet significant effect on protection against oxidative stress at low pH. A heme-dependent peroxidase, it probably does not have deferrochelatase activity. Converts guaiacol and H2O2 to tetraguaiacol, also acts on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Retains peroxidase activity when encapsulated but has . |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| eggNOG description | Dyp-type peroxidase |
| Orthologous group | COG2837 |
| KEGG orthology |
K07223
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Dyp_perox_N | PF04261.19 | 8.6e-40 | 9–137 | Dyp-type peroxidase, N-terminal |
Dyp_perox_C | PF20628.4 | 7.1e-63 | 140–304 | Dyp-type peroxidase, C-terminal |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pepC (M18 family aminopeptidase), high confidence from genomic context alone (score 791 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0798c cfp29 hyp |
hypothetical protein | 994 | 972 ctx | neighborhood:881 cooccurence:758 textmining:803 |
Rv0800 pepC |
M18 family aminopeptidase | 791 | 791 ctx | neighborhood:788 |
Rv0801 hyp |
hypothetical protein | 756 | 747 ctx | neighborhood:744 |
Rv3584 lpqE |
lipoprotein LpqE | 481 | 463 | coexpression:419 |
Rv0803 purL |
phosphoribosylformylglycinamidine synthase 2 | 445 | 445 ctx | neighborhood:442 |
Rv1224 tatB |
Sec-independent protein translocase protein TatB | 822 | 435 | coexpression:403 textmining:698 |
Rv2094c tatA |
Sec-independent protein translocase membrane-bound protein TatA | 495 | 433 | coexpression:401 |
Rv2093c tatC |
Sec-independent protein translocase transmembrane protein TatC | 781 | 283 | textmining:707 |
Rv0669c |
neutral ceramidase | 879 | 108 | textmining:870 |
Rv3432c gadB |
glutamate decarboxylase GadB | 452 | 77 | textmining:431 |
Rv2677c hemY |
protoporphyrinogen oxidase | 522 | 49 | textmining:518 |
Rv3607c folB |
dihydroneopterin aldolase | 405 | 46 | textmining:402 |
Rv2149c yfiH |
laccase domain-containing protein | 439 | 45 | textmining:437 |
Rv2503c scoB |
succinyl-CoA:3-ketoacid-CoA transferase subunit B | 432 | 41 | textmining:432 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: Dyp-type peroxidase
- Pfam (hmmscan --cut_ga): Dyp_perox_N PF04261.19 (E=9e-40), Dyp_perox_C PF20628.4 (E=7e-63)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215314.1)
- Domains: Pfam-A via hmmscan --cut_ga — Dyp_perox_N (PF04261.19), Dyp_perox_C (PF20628.4)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2837 - Curated reference: UniProt I6Y4U9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
14 functional partner(s); context anchor
pepC - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000848|Rv0799c| MAVPAVSPQPILAPLTPAAIFLVATIGADGEATVHDALSKISGLVRAIGFRDPTKHLSVVVSIGSDAWDRLFAGPRPTELHPFVELTGPRHTAPATPGDLLFHIRAETMDVCFELAGRILKSMGDAVTVVDEVHGFRFFDNRDLLGFVDGTENPSGPIAIKATTIGDEDRNFAGSCYVHVQKYVHDMASWESLSVTEQERVIGRTKLDDIELDDNAKPANSHVALNVITDDDGTERKIVRHNMPFGEVGKGEYGTYFIGYSRTPTVTEQMLRNMFLGDPAGNTDRVLDFSTAVTGGLFFSPTIDFLDHPPPLPQAATPTLAAGSLSIGSLKGSPR