Rv0802c Family assigned · medium auto-curated
H37Rv Rv0802c · MTBC0 mtbc0_000851 ·
218 aa · 898039–898695 (-) ·
RefSeq NP_215317.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | succinyl-CoA transferase |
|---|---|
| MTBC0 PGAP re-annotation | GNAT family protein |
| Revised (this work) | GNAT family protein. Pfam: Acetyltransf_3 (PF13302.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WQG7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Acetyl- and succinyl-CoA transferase Rv0802c |
| EC (curated) |
EC 2.8.1.-
|
| Curated function | Acetylates and succinylates nucleoid-associated, DNA-binding protein HupB. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
J Translation, ribosomal structure and biogenesis
|
|---|---|
| eggNOG description | Acetyltransferase (GNAT) domain |
| Orthologous group | COG1670 |
| Gene Ontology (50) |
GO:0003674, GO:0003824, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0006104, GO:0006139, GO:0006163, GO:0006637, GO:0006725, GO:0006732 +38 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.338 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acetyltransf_3 | PF13302.14 | 6.7e-13 | 16–164 | Acetyltransferase (GNAT) domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: purL (phosphoribosylformylglycinamidine synthase 2), high confidence from genomic context alone (score 725 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0803 purL |
phosphoribosylformylglycinamidine synthase 2 | 725 | 725 ctx | neighborhood:725 |
Rv0738 hyp |
hypothetical protein | 546 | 546 ctx | cooccurence:542 |
Rv2925c rnc |
ribonuclease III | 542 | 542 | coexpression:540 |
Rv2378c mbtG |
L-lysine N6-monooxygenase | 562 | 522 | coexpression:506 |
Rv3225c |
GCN5-like N-acetyltransferase | 512 | 512 | |
Rv0804 hyp |
hypothetical protein | 501 | 501 ctx | neighborhood:500 |
Rv1727 hyp |
hypothetical protein | 422 | 422 ctx | cooccurence:418 |
Rv3660c ssd hyp |
hypothetical protein | 416 | 416 ctx | cooccurence:416 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: succinyl-CoA transferase
- MTBC0 PGAP product: GNAT family protein
- Pfam (hmmscan --cut_ga): Acetyltransf_3 PF13302.14 (E=7e-13)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215317.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acetyltransf_3 (PF13302.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1670 - Curated reference: UniProt P9WQG7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
8 functional partner(s); context anchor
purL - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000851|Rv0802c| MSRHWPLFDLRITTPRLQLQLPTEELCDQLIDTILEGVHDPDRMPFSVPWTRASREDLPFNTLSHLWQQLAGFKRDDWSLPLAVLVDGRAVGVQALSSKDFPITRQVDSGSWLGLRYQGHGYGTEMRAAVLYFAFAELEAQVATSRSFVDNPASIAVSRRNGYRDNGLDRVAREGAMAEALLFRLTRDDWQRHRTVEVRVDGFDRCRPLFGPLEPPRY