yfiH Family assigned · medium auto-curated
H37Rv Rv2149c · MTBC0 - ·
250 aa · 2407622–2408374 (-) ·
RefSeq NP_216665.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | laccase domain-containing protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Laccase domain-containing protein. Pfam: Cu-oxidase_4 (PF02578.21). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WKD5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Purine nucleoside phosphorylase Rv2149c |
| EC (curated) |
EC 2.4.2.1, EC 2.4.2.28, EC 3.5.4.4
|
| Curated function | Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine and hypoxanthine. Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate. Also has adenosine deaminase activity. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | yfiH |
| eggNOG description | Belongs to the multicopper oxidase YfiH RL5 family |
| Orthologous group | COG1496 |
| KEGG orthology |
K05810
|
| Gene Ontology (13) |
GO:0003674, GO:0005488, GO:0005507, GO:0005575, GO:0005618, GO:0005623, GO:0030312, GO:0043167, GO:0043169, GO:0044464, GO:0046872, GO:0046914 +1 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Cu-oxidase_4 | PF02578.21 | 1.9e-74 | 25–248 | Multi-copper polyphenol oxidoreductase laccase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ftsZ (cell division protein FtsZ), high confidence from genomic context alone (score 886 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2148c hyp |
hypothetical protein | 978 | 960 ctx | neighborhood:882 coexpression:672 textmining:496 |
Rv2150c ftsZ |
cell division protein FtsZ | 899 | 886 ctx | neighborhood:879 |
Rv2147c sepF |
cell division protein SepF | 968 | 828 ctx | neighborhood:816 textmining:823 |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 803 | 804 ctx | neighborhood:734 |
Rv2154c ftsW |
lipid II flippase FtsW | 915 | 770 ctx | neighborhood:707 textmining:645 |
Rv2153c murG |
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase | 904 | 767 ctx | neighborhood:715 textmining:607 |
Rv2151c ftsQ |
cell division protein FtsQ | 740 | 729 ctx | neighborhood:715 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 704 | 677 ctx | neighborhood:605 |
Rv2157c murF |
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase | 668 | 667 ctx | neighborhood:611 |
Rv2158c murE |
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | 606 | 606 ctx | neighborhood:604 |
Rv2146c |
transmembrane protein | 623 | 598 ctx | neighborhood:571 |
Rv2155c murD |
UDP-N-acetylmuramoylalanine--D-glutamate ligase | 804 | 575 ctx | neighborhood:559 textmining:558 |
Rv2145c wag31 |
cell wall synthesis protein Wag31 | 466 | 446 ctx | neighborhood:423 |
Rv2160A |
Rv2160A, len: 211 aa. Conserved hypothetical protein, possibly a TetR-family transcriptional regulator,similar to N-terminal half of AL51266 | 435 | 435 ctx | neighborhood:420 |
Rv2159c hyp |
hypothetical protein | 426 | 426 ctx | neighborhood:420 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): laccase domain-containing protein
- Pfam (hmmscan --cut_ga): Cu-oxidase_4 PF02578.21 (E=2e-74)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216665.1)
- Domains: Pfam-A via hmmscan --cut_ga — Cu-oxidase_4 (PF02578.21)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1496 - Curated reference: UniProt P9WKD5 (SwissProt, reviewed; Evidence at protein level)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
26 functional partner(s); context anchor
ftsZ - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2149c|yfiH MLASTRHIARGDTGNVSVRIRRVTTTRAGGVSAPPFDTFNLGDHVGDDPAAVAANRARLAAAIGLPGNRVVWMNQVHGDRVELVDQPRNTALDDTDGLVTATPRLALAVVTADCVPVLMADARAGIAAAVHAGRAGAQRGVVVRALEVMLSLGAQVRDISALLGPAVSGRNYEVPAAMADEVEAALPGSRTTTAAGTPGVDLRAGIACQLRDLGVESIDVDPRCTVADPTLFSHRRDAPTGRFASLVWME