tatA Family assigned · medium auto-curated
H37Rv Rv2094c · MTBC0 mtbc0_002228 ·
83 aa · 2381670–2381921 (-) ·
RefSeq NP_216610.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | Sec-independent protein translocase membrane-bound protein TatA |
|---|---|
| MTBC0 PGAP re-annotation | Sec-independent protein translocase subunit TatA |
| Revised (this work) | Sec-independent protein translocase subunit TatA. Pfam: TatA_B_E (PF02416.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WGA1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Sec-independent protein translocase protein TatA |
| Curated function | Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
U Intracellular trafficking, secretion and vesicular transport
|
|---|---|
| Preferred name | tatA |
| eggNOG description | Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system |
| Orthologous group | COG1826 |
| KEGG orthology |
K03116
|
| KEGG pathways |
map03060, map03070
|
| KEGG modules |
M00336
|
| Gene Ontology (2) |
GO:0008150, GO:0040007
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
TatA_B_E | PF02416.22 | 1.6e-17 | 4–49 | mttA/Hcf106 family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: tatC (Sec-independent protein translocase transmembrane protein TatC), high confidence from genomic context alone (score 983 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2093c tatC |
Sec-independent protein translocase transmembrane protein TatC | 994 | 983 ctx | neighborhood:787 coexpression:706 experimental:629 textmining:668 |
Rv3396c guaA |
GMP synthase | 935 | 933 | coexpression:921 |
Rv2097c pafA |
proteasome accessory factor PafA | 824 | 801 ctx | neighborhood:796 |
Rv2096c pafB |
proteasome accessory factor B | 809 | 799 ctx | neighborhood:796 |
Rv2095c pafC |
proteasome accessory factor C | 807 | 797 ctx | neighborhood:796 |
Rv2092c helY |
ATP-dependent DNA helicase HelY | 674 | 675 ctx | neighborhood:673 |
Rv2091c |
membrane protein | 533 | 533 ctx | neighborhood:508 |
Rv1337 |
integral membrane protein | 542 | 475 | experimental:467 |
Rv2098c PE_PGRS36 |
PE-PGRS family protein PE_PGRS36; Rv2098c, (MTCY49.38c), len: 434 aa. PE_PGRS36,Member of the Mycobacterium tuberculosis PE family, PGRS sub | 500 | 474 ctx | neighborhood:471 |
Rv2099c PE21 |
Rv2099c, (MTCY49.39c), len: 58 aa. PE21, Member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002); 5'-end of Rv2098 | 497 | 471 ctx | neighborhood:471 |
Rv0110 |
integral membrane protein | 575 | 469 | experimental:467 |
Rv2146c |
transmembrane protein | 467 | 468 ctx | cooccurence:429 |
Rv2122c hisE |
phosphoribosyl-ATP pyrophosphatase | 463 | 463 | |
Rv0846c mmcO |
oxidase | 556 | 433 | experimental:415 |
Rv0799c hyp |
hypothetical protein | 495 | 433 | coexpression:401 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: Sec-independent protein translocase membrane-bound protein TatA
- MTBC0 PGAP product: Sec-independent protein translocase subunit TatA
- Pfam (hmmscan --cut_ga): TatA_B_E PF02416.22 (E=2e-17)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216610.1)
- Domains: Pfam-A via hmmscan --cut_ga — TatA_B_E (PF02416.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1826 - Curated reference: UniProt P9WGA1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
35 functional partner(s); context anchor
tatC - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002228|Rv2094c|tatA MGSLSPWHWAILAVVVIVLFGAKKLPDAARSLGKSLRIFKSEVRELQNENKAEASIETPTPVQSQRVDPSAASGQDSTEARPA