mftG Family assigned · medium auto-curated
H37Rv Rv0697 · MTBC0 mtbc0_000738 ·
479 aa · 801019–802458 (+) ·
RefSeq NP_215211.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | dehydrogenase |
|---|---|
| MTBC0 PGAP re-annotation | mycofactocin system GMC family oxidoreductase MftG |
| Revised (this work) | Mycofactocin system GMC family oxidoreductase MftG. Pfam: GMC_oxred_N (PF00732.26), GMC_oxred_C (PF05199.20). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6Y8H4
TrEMBL · unreviewed
· Inferred from homology
|
|---|---|
| UniProt name | Probable dehydrogenase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
E Amino acid transport and metabolism
|
|---|---|
| eggNOG description | Dehydrogenase |
| Orthologous group | COG2303 |
| EC number |
EC 1.1.99.1
|
| KEGG orthology |
K00108
|
| KEGG pathways |
map00260, map01100
|
| KEGG modules |
M00555
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.358 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
GMC_oxred_N | PF00732.26 | 8.4e-32 | 9–298 | GMC oxidoreductase |
GMC_oxred_C | PF05199.20 | 6.6e-30 | 358–471 | GMC oxidoreductase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mftF (mycofactocin biosynthesis glycosyltransferase MftF), high confidence from genomic context alone (score 967 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0696 mftF |
mycofactocin biosynthesis glycosyltransferase MftF | 968 | 967 ctx | neighborhood:881 coexpression:731 |
Rv0695 mftE |
mycofactocin system creatinine amidohydrolase family protein MftE | 845 | 845 ctx | neighborhood:813 |
Rv2194 qcrC |
ubiquinol-cytochrome C reductase cytochrome subunit C | 785 | 775 | experimental:762 |
Rv3529c hyp |
hypothetical protein | 758 | 748 | experimental:411 database:583 |
Rv2267c stf3 hyp |
hypothetical protein | 755 | 745 | experimental:411 database:583 |
Rv1691 hyp |
hypothetical protein | 754 | 744 | experimental:411 database:583 |
Rv0694 mftD |
mycofactocin system heme/flavin oxidoreductase MftD | 756 | 736 ctx | neighborhood:702 |
Rv0832 PE_PGRS12 |
PE-PGRS family protein PE_PGRS12 | 739 | 729 | database:573 |
Rv3812 PE_PGRS62 |
PE-PGRS family protein PE_PGRS62 | 739 | 729 | database:573 |
Rv2328 PE23 |
PE family protein PE23 | 739 | 729 | database:573 |
Rv3036c TB22.2 hyp |
hypothetical protein | 739 | 729 | database:573 |
Rv0693 mftC |
mycofactocin radical SAM maturase MftC | 718 | 706 ctx | neighborhood:702 |
Rv0692 mftB |
mycofactocin system protein MftB | 716 | 705 ctx | neighborhood:702 |
Rv3492c |
Mce associated protein | 698 | 686 | database:536 |
Rv1972 |
Mce associated membrane protein | 695 | 684 | database:536 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: dehydrogenase
- MTBC0 PGAP product: mycofactocin system GMC family oxidoreductase MftG
- Pfam (hmmscan --cut_ga): GMC_oxred_N PF00732.26 (E=8e-32), GMC_oxred_C PF05199.20 (E=7e-30)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215211.1)
- Domains: Pfam-A via hmmscan --cut_ga — GMC_oxred_N (PF00732.26), GMC_oxred_C (PF05199.20)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2303 - Curated reference: UniProt I6Y8H4 (TrEMBL, unreviewed; Inferred from homology)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
27 functional partner(s); context anchor
mftF - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000738|Rv0697|mftG MTAAVRHSDVLVVGAGSAGSVVAERLSMDSSCVVTVLEAGPGLADPGLLAQTANGLQLPIGAGSPLVERYRTRLTDRPVRHLPIVRGATVGGSGAINGGYFCRGLPSDFDRASIPGWAWSDVLEHFRAIETDLDFETPVHGRSGPIPVRRTHEMTGITESFMAAAEDAGFAWIADLNDVGPEMPSGVGAVPLNIVNGVRTSSAVGYLMPALGRPNLTLLARTRAVRLRFSATTAVGVDAIGPGGPVSLSADRIVLCAGAIQSAHLLMLSGVGEEEVLRSAGVKVLMALPVGMGCSDHPEWVMPTNWAVAVDRPVLEVLLSTHDGIEIRPYTGGFVAMTGDGTAGHRDWPHIGVALMQPRARGRITLVSSDPQIPVRIEHRYDSEPADVAALRQGSALAHELCGAATRIGPAVWATSQHLCGSAPMGTDDDPRAVVDPRCRVRGIENLWVIDGSVLPSITSRGPHATIVMLGHRAAEFVQ