Rv0361 Family assigned · low
H37Rv Rv0361 · MTBC0 mtbc0_000381 ·
275 aa · 442184–443011 (+) ·
RefSeq NP_214875.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | membrane protein |
|---|---|
| MTBC0 PGAP re-annotation | hypothetical protein |
| Revised (this work) | Cell-division / septum-assembly-associated membrane protein (eggNOG COG3115, COG category D 'cell septum assembly'). RefSeq leaves it a 'conserved membrane protein'. Pathway-level role in division; molecular detail undetermined. |
Curated reference (UniProt)
| UniProt |
O06311
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable conserved membrane protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| eggNOG description | cell septum assembly |
| Orthologous group | COG3115 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mgtE (Mg2+ transport transmembrane protein MgtE), medium confidence from genomic context alone (score 467 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0360c hyp |
hypothetical protein | 726 | 726 ctx | neighborhood:726 |
Rv0362 mgtE |
Mg2+ transport transmembrane protein MgtE | 467 | 467 ctx | neighborhood:464 |
Rv0178 |
Mce associated membrane protein | 631 | 52 | textmining:627 |
Rv3069 crcB |
fluoride ion transporter CrcB | 870 | 47 | textmining:870 |
Rv0200 |
transmembrane protein | 804 | 47 | textmining:803 |
Rv1733c |
transmembrane protein | 492 | 47 | textmining:489 |
Rv0418 lpqL |
lipoprotein aminopeptidase LpqL | 658 | 46 | textmining:657 |
Rv0556 |
transmembrane protein | 870 | 45 | textmining:870 |
Rv0835 lpqQ |
lipoprotein LpqQ | 860 | 44 | textmining:860 |
Rv1280c oppA |
oligopeptide ABC transporter substrate-binding lipoprotein OppA | 514 | 42 | textmining:514 |
Rv1022 lpqU |
lipoprotein LpqU | 870 | 41 | textmining:870 |
Rv3630 |
integral membrane protein | 861 | 41 | textmining:861 |
Rv0403c mmpS1 |
membrane protein MmpS1 | 750 | 41 | textmining:750 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- eggNOG COG3115 = cell septum assembly (COG category D)
- eggNOG-mapper orthology (COG functional assignment, under-propagated by the auto-curation). A family/activity-level assignment from orthology, not a substrate demonstrated in M. tuberculosis.
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214875.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3115 - Curated reference: UniProt O06311 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
13 functional partner(s); context anchor
mgtE - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000381|Rv0361| MSNAPEPDRSAGESGSEPAGERSADPGEERTESYPLVPHDAETETVVITTSDNDAAVTQPEAQRERRFTAPGFDAKETQVIVTAHEAATEVFQTNQAPTTPPRMPTGMPPKTAVPQSIPPRTEATSVRQRTWGWALAVVVIVLALAAIAILGTVLLTRGKHSKMSQEDQVRQAIQSLDIAIQTGDLTALRSLTCGSTRDGYVDYDERDWAETYRRVSAAKQYPVIASIDQVVVNGAHAEANVTTFMAFDPQVRSTRSLDLQFRDDQWKICQSSSN