mgtE Resolved · high auto-curated
H37Rv Rv0362 · MTBC0 mtbc0_000382 ·
460 aa · 443233–444615 (+) ·
RefSeq NP_214876.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | Mg2+ transport transmembrane protein MgtE |
|---|---|
| MTBC0 PGAP re-annotation | magnesium transporter |
| Revised (this work) | Magnesium transporter. Pfam: MgtE_N (PF03448.23), CBS (PF00571.34), MgtE (PF01769.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06312
TrEMBL · unreviewed
· Inferred from homology
|
|---|---|
| UniProt name | Magnesium transporter MgtE |
| Curated function | Acts as a magnesium transporter. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | mgtE |
| eggNOG description | Acts as a magnesium transporter |
| Orthologous group | COG2239 |
| KEGG orthology |
K06213
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.432 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 7 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MgtE_N | PF03448.23 | 5.5e-18 | 37–134 | MgtE intracellular N domain |
CBS | PF00571.34 | 2.3e-06 | 204–256 | CBS domain |
MgtE | PF01769.22 | 1.6e-30 | 326–449 | Divalent cation transporter |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0361 (membrane protein), medium confidence from genomic context alone (score 467 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0361 |
membrane protein | 467 | 467 ctx | neighborhood:464 |
Rv1992c ctpG |
cation transporter ATPase G | 452 | 187 | |
Rv0924c mntH |
divalent metal cation transporter MntH | 615 | 129 | textmining:576 |
Rv0655 mkl |
ABC transporter ATP-binding protein | 467 | 98 | textmining:434 |
Rv1272c |
drug ABC transporter ATP-binding protein | 563 | 77 | textmining:547 |
Rv2127 ansP1 |
L-asparagine permease | 495 | 42 | textmining:495 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: Mg2+ transport transmembrane protein MgtE
- MTBC0 PGAP product: magnesium transporter
- Pfam (hmmscan --cut_ga): MgtE_N PF03448.23 (E=6e-18), CBS PF00571.34 (E=2e-06), MgtE PF01769.22 (E=2e-30)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214876.1)
- Domains: Pfam-A via hmmscan --cut_ga — MgtE_N (PF03448.23), CBS (PF00571.34), MgtE (PF01769.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2239 - Curated reference: UniProt O06312 (TrEMBL, unreviewed; Inferred from homology)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
6 functional partner(s); context anchor
Rv0361 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000382|Rv0362|mgtE MSIRPAENSTLDIRHVIGIGTPKAVDLWLDVVTELPDRARELGSLSKAELGKLGPLLDGTNAVELFESIDDKLAAEALHAMDPSLAATFLEALDSDHAANILREFKEPKREALLTLLPLERAMVLRGLLSWPEDCAAAHMVPETLTVRPNMTVSQAVASVRERASGLRSDARTTAYVYVTDADSHLLGVIAFRALVLANPEQRVRELMGDDLIVVSPLTDKELAAQTIMGHNLMAVPVVDADNRLLGIIAEDEAIDIAEEEATEDAERQGGSAPLEVPYLRASPWLLWRKRVVWLLVLFAAEAYTGSVLRAFSDEMEAVIALAFFIPLLIGTGGNTGTQIATTLVRAMATGQVRFRDVPAVLAKELSTGVLVGLTMAAAAVVRAWTLGVGPQVTLTVALTVAAIVVWSSLVAAVLPPLLKKLRIDPAIVSGPMIATIVDGTGLLIYFLVAHLTLTELHGL