Rv1733c Family assigned · medium auto-curated
H37Rv Rv1733c · MTBC0 mtbc0_001845 ·
210 aa · 1971927–1972559 (-) ·
RefSeq NP_216249.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane protein |
|---|---|
| MTBC0 PGAP re-annotation | hypothetical protein |
| Revised (this work) | Contains Rv1733c (PF27099.1) domain(s); putative function inferred from the domain architecture. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WLS9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable membrane protein Rv1733c |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| Orthologous group | 2E3P4 |
|---|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.979 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 8 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Rv1733c | PF27099.1 | 1.6e-12 | 83–152 | Rv1733c-like dormancy-induced membrane protein |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3134c (universal stress protein), high confidence from genomic context alone (score 937 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3134c |
universal stress protein | 975 | 937 ctx | cooccurence:647 coexpression:829 textmining:626 |
Rv2032 acg |
NAD(P)H nitroreductase | 939 | 882 | coexpression:860 textmining:510 |
Rv2627c hyp |
hypothetical protein | 876 | 877 | coexpression:860 |
Rv2626c hrp1 |
hypoxic response protein | 950 | 861 | coexpression:860 textmining:659 |
Rv2030c hyp |
hypothetical protein | 881 | 860 | coexpression:860 |
Rv1737c narK2 |
nitrate/nitrite transporter | 872 | 860 | coexpression:860 |
Rv1738 hyp |
hypothetical protein | 922 | 845 | coexpression:797 textmining:518 |
Rv2623 TB31.7 |
universal stress protein | 878 | 843 | coexpression:843 |
Rv1996 |
universal stress protein | 870 | 843 | coexpression:822 |
Rv2031c hspX |
alpha-crystallin | 936 | 837 | coexpression:804 textmining:625 |
Rv3127 hyp |
hypothetical protein | 902 | 836 | coexpression:813 textmining:432 |
Rv1736c narX |
nitrate reductase-like protein NarX | 836 | 836 | coexpression:836 |
Rv2028c |
universal stress protein | 881 | 833 | coexpression:740 |
Rv2625c rip3 |
zinc metalloprotease Rip3 | 832 | 833 | coexpression:810 |
Rv3131 |
NAD(P)H nitroreductase | 898 | 828 | coexpression:806 textmining:433 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane protein
- MTBC0 PGAP product: hypothetical protein
- Pfam (hmmscan --cut_ga): Rv1733c PF27099.1 (E=2e-12)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216249.1)
- Domains: Pfam-A via hmmscan --cut_ga — Rv1733c (PF27099.1)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2E3P4 - Curated reference: UniProt P9WLS9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
92 functional partner(s); context anchor
Rv3134c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001845|Rv1733c| MIATTRDREGATMITFRLRLPCRTILRVFSRNPLVRGTDRLEAVVMLLAVTVSLLTIPFAAAAGTAVHDSRSHVYAHQAQTRHPATATVIDHEGVIDSNTTATSAPPRTKITVPARWVVNGIERSGEVNAKPGTKSGDRVGIWVDSAGQLVDEPAPPARAIADAALAALGLWLSVAAVAGALLALTRAILIRVRNASWQHDIDSLFCTQR