fba Resolved · high auto-curated
H37Rv Rv0363c · MTBC0 mtbc0_000383 ·
344 aa · 444627–445661 (-) ·
RefSeq NP_214877.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | fructose-bisphosphate aldolase |
|---|---|
| MTBC0 PGAP re-annotation | class II fructose-bisphosphate aldolase |
| Revised (this work) | Class II fructose-bisphosphate aldolase. Pfam: F_bP_aldolase (PF01116.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WQA3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Fructose-bisphosphate aldolase |
| EC (curated) |
EC 4.1.2.13
|
| Curated function | Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
G Carbohydrate transport and metabolism
|
|---|---|
| Preferred name | fba |
| eggNOG description | fructose-bisphosphate aldolase |
| Orthologous group | COG0191 |
| EC number |
EC 4.1.2.13
|
| KEGG orthology |
K01624
|
| KEGG pathways |
map00010, map00030, map00051, map00680, map00710, map01100, map01110, map01120, map01130, map01200, map01230
|
| KEGG modules |
M00001, M00003, M00165, M00167, M00344, M00345
|
| Gene Ontology (145) |
GO:0003674, GO:0003824, GO:0004332, GO:0005488, GO:0005515, GO:0005575, GO:0005576, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829 +133 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.515 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
F_bP_aldolase | PF01116.27 | 4.8e-81 | 9–340 | Fructose-bisphosphate aldolase class-II |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1436 gap |
glyceraldehyde 3-phosphate dehydrogenase | 992 | 982 | coexpression:793 database:900 textmining:573 |
Rv1438 tpi |
triosephosphate isomerase | 995 | 977 | coexpression:763 database:900 textmining:831 |
Rv1023 eno |
enolase | 986 | 964 | coexpression:802 database:800 textmining:636 |
Rv0946c pgi |
glucose-6-phosphate isomerase | 978 | 949 | coexpression:735 database:800 textmining:596 |
Rv3010c pfkA |
6-phosphofructokinase | 977 | 948 | database:900 textmining:595 |
Rv1449c tkt |
transketolase | 973 | 948 | coexpression:461 database:900 textmining:511 |
Rv1448c tal |
transaldolase | 981 | 945 | coexpression:431 database:900 textmining:682 |
Rv2029c pfkB |
6-phosphofructokinase PfkB | 967 | 939 | database:900 textmining:481 |
Rv0489 gpm1 |
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase | 953 | 922 | coexpression:624 database:800 textmining:429 |
Rv1099c glpX |
fructose 1,6-bisphosphatase | 947 | 915 | database:900 textmining:414 |
Rv0727c fucA |
L-fuculose phosphate aldolase FucA | 924 | 909 | database:900 |
Rv1617 pykA |
pyruvate kinase | 943 | 906 | coexpression:440 database:800 textmining:420 |
Rv0478 deoC |
2-deoxyribose-5-phosphate aldolase | 915 | 902 | database:900 |
Rv1437 pgk |
phosphoglycerate kinase | 958 | 884 | coexpression:827 textmining:653 |
Rv2241 aceE |
pyruvate dehydrogenase E1 component | 877 | 826 | database:800 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: fructose-bisphosphate aldolase
- MTBC0 PGAP product: class II fructose-bisphosphate aldolase
- Pfam (hmmscan --cut_ga): F_bP_aldolase PF01116.27 (E=5e-81)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214877.1)
- Domains: Pfam-A via hmmscan --cut_ga — F_bP_aldolase (PF01116.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0191 - Curated reference: UniProt P9WQA3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 133 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000383|Rv0363c|fba MPIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFGSGLGVKDMVTGAVALAEFTHVIAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKGGNPLFQSHMWDGSAVPIDENLAIAQELLKAAAAAKIILEIEIGVVGGEEDGVANEINEKLYTSPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGLPADAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVDTDTQYAFTRPIAGHMFTNYDGVLKVDGEVGVKKVYDPRSYLKKAEASMSQRVVQACNDLHCAGKSLTH