fabG4 Resolved · high auto-curated
H37Rv Rv0242c · MTBC0 mtbc0_000258 ·
454 aa · 291046–292410 (-) ·
RefSeq NP_214756.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | 3-oxoacyl-ACP reductase FabG |
|---|---|
| MTBC0 PGAP re-annotation | 3-oxoacyl-ACP reductase |
| Revised (this work) | 3-oxoacyl-ACP reductase. Pfam: adh_short (PF00106.32), KR (PF08659.17), adh_short_C2 (PF13561.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6Y778
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | 3-oxoacyl-[acyl-carrier-protein] reductase [NADH] |
| EC (curated) |
EC 1.1.1.212
|
| Curated function | Catalyzes the NADH-dependent reduction of beta-ketoacyl derivatives. Can accept the beta-oxo fatty acyl group covalently linked with CoA or ACP for catalysis. Highly specific for NADH. Could be involved in fatty acid biosynthesis (Probable). |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolismQ Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| Preferred name | fabG |
| eggNOG description | Catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis |
| Orthologous group | COG1028 |
| EC number |
EC 1.1.1.100
|
| KEGG orthology |
K00059
|
| KEGG pathways |
map00061, map00333, map00780, map01040, map01100, map01130, map01212
|
| KEGG modules |
M00083, M00572
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.223 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 10 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
adh_short | PF00106.32 | 5.7e-45 | 214–401 | short chain dehydrogenase |
KR | PF08659.17 | 2.0e-13 | 216–373 | KR domain |
adh_short_C2 | PF13561.13 | 2.3e-47 | 220–447 | Enoyl-(Acyl carrier protein) reductase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: htdX (3-hydroxyacyl-thioester dehydratase HtdX), high confidence from genomic context alone (score 997 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0241c htdX |
3-hydroxyacyl-thioester dehydratase HtdX | 998 | 997 ctx | neighborhood:879 cooccurence:768 coexpression:853 textmining:449 |
Rv0243 fadA2 |
acetyl-CoA acetyltransferase FadA | 992 | 992 ctx | neighborhood:773 cooccurence:740 coexpression:863 |
Rv2524c fas |
fatty acid synthase | 981 | 979 | coexpression:508 experimental:475 database:918 |
Rv2243 fabD |
malonyl CoA-acyl carrier protein transacylase | 960 | 959 | database:900 |
Rv3389c htdY |
3-hydroxyacyl-thioester dehydratase HtdY | 951 | 931 ctx | fusion:900 |
Rv3538 |
dehydrogenase | 931 | 931 ctx | fusion:899 |
Rv3559c |
oxidoreductase | 913 | 914 | database:900 |
Rv0769 |
oxidoreductase | 913 | 914 | database:900 |
Rv1350 fabG2 |
3-oxoacyl-ACP reductase FabG | 918 | 913 | database:900 |
Rv0649 fabD2 |
malonyl CoA-acyl carrier protein transacylase | 900 | 900 | database:900 |
Rv0148 |
short-chain type dehydrogenase/reductase | 899 | 899 ctx | fusion:899 |
Rv3085 sadH |
oxidoreductase SadH | 680 | 681 ctx | cooccurence:672 |
Rv1543 |
oxidoreductase | 612 | 598 ctx | cooccurence:540 |
Rv3140 fadE23 |
acyl-CoA dehydrogenase FadE23 | 590 | 556 | |
Rv1937 |
oxygenase | 591 | 543 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: 3-oxoacyl-ACP reductase FabG
- MTBC0 PGAP product: 3-oxoacyl-ACP reductase
- Pfam (hmmscan --cut_ga): adh_short PF00106.32 (E=6e-45), KR PF08659.17 (E=2e-13), adh_short_C2 PF13561.13 (E=2e-47)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214756.1)
- Domains: Pfam-A via hmmscan --cut_ga — adh_short (PF00106.32), KR (PF08659.17), adh_short_C2 (PF13561.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1028 - Curated reference: UniProt I6Y778 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
111 functional partner(s); context anchor
htdX - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000258|Rv0242c|fabG4 MAPKRSSDLFSQVVNSGPGSFLARQLGVPQPETLRRYRAGEPPLTGSLLIGGAGRVVEPLRAALEKDYDLVGNNLGGRWADSFGGLVFDATGITEPAGLKGLHEFFTPVLRNLGRCGRVVVVGGTPEAAASTNERIAQRALEGFTRSLGKELRRGATTALVYLSPDAKPAATGLESTMRFLLSAKSAYVDGQVFSVGADDSTPPADWEKPLDGKVAIVTGAARGIGATIAEVFARDGAHVVAIDVESAAENLAETASKVGGTALWLDVTADDAVDKISEHLRDHHGGKADILVNNAGITRDKLLANMDDARWDAVLAVNLLAPLRLTEGLVGNGSIGEGGRVIGLSSIAGIAGNRGQTNYATTKAGMIGITQALAPGLAAKGITINAVAPGFIETQMTAAIPLATREVGRRLNSLLQGGQPVDVAEAIAYFASPASNAVTGNVIRVCGQAMIGA