Rv0247c Family assigned · medium auto-curated
H37Rv Rv0247c · MTBC0 mtbc0_000263 ·
248 aa · 298498–299244 (-) ·
RefSeq NP_214761.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | succinate dehydrogenase iron-sulfur subunit |
|---|---|
| MTBC0 PGAP re-annotation | succinate dehydrogenase/fumarate reductase iron-sulfur subunit |
| Revised (this work) | Succinate dehydrogenase/fumarate reductase iron-sulfur subunit. Pfam: Fer2_3 (PF13085.12), Fer2 (PF00111.33), Fer4_8 (PF13183.12). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O53669
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable succinate dehydrogenase [iron-sulfur subunit] |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversion
|
|---|---|
| Preferred name | sdhB_1 |
| eggNOG description | fumarate reductase |
| Orthologous group | COG0479 |
| EC number |
EC 1.3.5.1, EC 1.3.5.4
|
| KEGG orthology |
K00240
|
| KEGG pathways |
map00020, map00190, map00650, map00720, map01100, map01110, map01120, map01130, map01200
|
| KEGG modules |
M00009, M00011, M00149, M00173, M00374, M00376
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.144 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Fer2_3 | PF13085.12 | 1.6e-24 | 8–105 | 2Fe-2S iron-sulfur cluster binding domain |
Fer2 | PF00111.33 | 3.0e-04 | 21–69 | 2Fe-2S iron-sulfur cluster binding domain |
Fer4_8 | PF13183.12 | 1.1e-08 | 141–216 | 4Fe-4S dicluster domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0249c (succinate dehydrogenase membrane anchor subunit), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0249c |
succinate dehydrogenase membrane anchor subunit | 999 | 1000 ctx | neighborhood:839 cooccurence:774 experimental:997 textmining:870 |
Rv0248c |
succinate dehydrogenase flavoprotein subunit | 999 | 1000 ctx | neighborhood:881 cooccurence:774 coexpression:882 experimental:997 database:956 textmining:852 |
Rv3316 sdhC |
succinate dehydrogenase cytochrome B-556 subunit | 999 | 998 | coexpression:696 experimental:788 database:962 textmining:861 |
Rv3317 sdhD |
succinate dehydrogenase hydrophobic membrane anchor subunit | 998 | 993 | coexpression:670 experimental:784 database:900 textmining:822 |
Rv1552 frdA |
fumarate reductase flavoprotein subunit | 995 | 991 | coexpression:484 experimental:454 database:956 textmining:483 |
Rv3318 sdhA |
succinate dehydrogenase flavoprotein subunit | 995 | 990 | coexpression:491 experimental:454 database:956 textmining:569 |
Rv0952 sucD |
succinyl-CoA ligase subunit alpha | 979 | 976 | coexpression:668 database:900 |
Rv0951 sucC |
succinyl-CoA ligase subunit beta | 981 | 975 | coexpression:656 database:900 |
Rv1554 frdC |
fumarate reductase membrane anchor subunit | 983 | 974 | coexpression:452 experimental:549 database:900 |
Rv1555 frdD |
fumarate reductase membrane anchor subunit | 981 | 972 | coexpression:417 experimental:549 database:900 |
Rv1098c fum |
fumarate hydratase | 956 | 951 | coexpression:445 database:900 |
Rv1248c kgd |
multifunctional 2-oxoglutarate dehydrogenase E1 component /2-oxoglutarate dehydrogenase dihydrolipoyllysine-residue succinyltransferase | 967 | 950 | coexpression:931 |
Rv1553 frdB |
fumarate reductase iron-sulfur subunit | 924 | 915 | database:900 |
Rv0234c gabD1 |
succinate-semialdehyde dehydrogenase | 915 | 912 | database:900 |
Rv1731 gabD2 |
succinate-semialdehyde dehydrogenase | 915 | 912 | database:900 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: succinate dehydrogenase iron-sulfur subunit
- MTBC0 PGAP product: succinate dehydrogenase/fumarate reductase iron-sulfur subunit
- Pfam (hmmscan --cut_ga): Fer2_3 PF13085.12 (E=2e-24), Fer2 PF00111.33 (E=3e-04), Fer4_8 PF13183.12 (E=1e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214761.1)
- Domains: Pfam-A via hmmscan --cut_ga — Fer2_3 (PF13085.12), Fer2 (PF00111.33), Fer4_8 (PF13183.12)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0479 - Curated reference: UniProt O53669 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
99 functional partner(s); context anchor
Rv0249c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000263|Rv0247c| MTYSASMRVWRGDESCGELREFTVEVNEGEVVLDVILRLQQTQTPDLAVRWNCKAGKCGSCSAEINGKPRLMCMTRMSTFDEDEIVTVTPMRTFPVIRDLVTDVSFNYQKAREIPSFAPPKELQPSEYRMAQVDVARSQEFRKCIECFLCQNVCHVVRDHEENKDAFAGPRFLMRIAELEMHPLDTRDRRSQAQEEHGLGYCNITKCCTEVCPENIKITDNALIPMKERVADRKYDPVVWLGSKLFRR