Rv3762c Resolved · high auto-curated
H37Rv Rv3762c · MTBC0 mtbc0_003986 ·
626 aa · 4231117–4232997 (-) ·
RefSeq NP_218279.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hydrolase |
|---|---|
| MTBC0 PGAP re-annotation | alkyl/aryl-sulfatase |
| Revised (this work) | Alkyl/aryl-sulfatase. Pfam: Lactamase_B (PF00753.34), Alkyl_sulf_dimr (PF14863.13), Alkyl_sulf_C (PF14864.12). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O69728
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Linear primary-alkylsulfatase |
| EC (curated) |
EC 3.1.6.21
|
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
Q Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| eggNOG description | alkyl sulfatase |
| Orthologous group | COG2015 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.389 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 11 synonymous, 13 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.26% of strains (375) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Lactamase_B | PF00753.34 | 4.7e-21 | 98–324 | Metallo-beta-lactamase superfamily |
Alkyl_sulf_dimr | PF14863.13 | 1.4e-60 | 361–499 | Alkyl sulfatase dimerisation |
Alkyl_sulf_C | PF14864.12 | 1.1e-32 | 510–626 | Alkyl sulfatase C-terminal |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: fadE36 (acyl-CoA dehydrogenase FadE36), medium confidence from genomic context alone (score 586 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3761c fadE36 |
acyl-CoA dehydrogenase FadE36 | 586 | 586 ctx | neighborhood:559 |
Rv3763 lpqH |
lipoprotein LpqH | 532 | 532 ctx | neighborhood:512 |
Rv0663 atsD |
arylsulfatase AtsD | 435 | 404 | |
Rv0712 hyp |
hypothetical protein | 889 | 187 | textmining:870 |
Rv2407 rnz |
ribonuclease Z | 888 | 166 | textmining:872 |
Rv3849 espR |
ESX-1 transcriptional regulator EspR | 443 | 59 | textmining:433 |
Rv1730c |
penicillin-binding protein | 661 | 45 | textmining:660 |
Rv2837c nrnA |
bifunctional oligoribonuclease/PAP phosphatase NrnA | 870 | 44 | textmining:870 |
Rv3205c hyp |
hypothetical protein | 870 | 41 | textmining:870 |
Rv0484c |
short-chain type oxidoreductase | 870 | 41 | textmining:870 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hydrolase
- MTBC0 PGAP product: alkyl/aryl-sulfatase
- Pfam (hmmscan --cut_ga): Lactamase_B PF00753.34 (E=5e-21), Alkyl_sulf_dimr PF14863.13 (E=1e-60), Alkyl_sulf_C PF14864.12 (E=1e-32)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218279.1)
- Domains: Pfam-A via hmmscan --cut_ga — Lactamase_B (PF00753.34), Alkyl_sulf_dimr (PF14863.13), Alkyl_sulf_C (PF14864.12)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2015 - Curated reference: UniProt O69728 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
10 functional partner(s); context anchor
fadE36 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003986|Rv3762c| MPMEHKPPTAVIQAAHGEHSLPLHDTTDFDDADRGFIAALSPCVIKAADGRVVWDNDAYSFLDGAAPTSVHPSLWRQSQLTAKQGLYQVVPGIYQVRGFDISNISFVEGDTGLIVIDPLVSTEVAAAALDLYRAHRGADRPVVAVIYTHSHVDHFGGVLGVTTQADVDAGKVAVLAPEGFTAHAVQENIYAGSAMMRRAGYMYGTVLARGLRGHVGCGLGQTLSTGEVSLVVPTVDITETGETHTIDGVEIEFQMAPGTEAPAEMHFYFPRFRALCMAENATHNLHNLLTLRGALVRDPRAWSGYLTEAIDTFADRTDVVFASHHWPTWGREKIVEFLSQQRDMYSYLHDQTLRLLNQGYTGVEIAEMFQLPPALQRAWHTHGYYGSVSHNVKAIYQRYMGWFDGNPGWLWPHPPEALAPRYVDALGGIDRVLELAREAFDAGDFRWAATLLDHAVFADSEHAAARGLYADTLEQLAYGAECATWRNFFLTGAAELRDGNPGSSGQVPAPTFFAQLTPDQIFDVLAISINGPRAWDLDLAIDFTFTEPDVNYRLTLRNGVLIHRKLPADPATANATVTVGDKVRLVAAALGDISSPGFEVFGDRTVLQTFLSVLDRPDSAFNIVTP