eis Resolved · high auto-curated
H37Rv Rv2416c · MTBC0 - ·
402 aa · 2714124–2715332 (-) ·
RefSeq NP_216932.2
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | enhanced intracellular survival protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Enhanced intracellular survival protein. Pfam: Acetyltransf_9 (PF13527.14), Acetyltransf_17 (PF17668.8), SCP2_2 (PF13530.12). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WFK7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | N-acetyltransferase Eis |
| EC (curated) |
EC 2.3.1.-
|
| Curated function | Effector that is released into the host cell and affects host immune responses; it negatively modulates inflammation, macrophage autophagy, and cell death through redox-dependent signaling. Acts as an acetyltransferase. Acetylates 'Lys-55' of dual-specificity protein phosphatase 16 (DUSP16)/mitogen-activated protein kinase phosphatase-7 (MKP-7), a JNK-specific phosphatase; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis. Inhibits Con A-mediated T-cell proliferation . |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | eis |
| eggNOG description | N-acetyltransferase Eis |
| Orthologous group | COG4552 |
| Gene Ontology (89) |
GO:0003674, GO:0003824, GO:0005488, GO:0005515, GO:0008080, GO:0008150, GO:0008152, GO:0009056, GO:0009605, GO:0009607, GO:0009987, GO:0010941 +77 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 2.505 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 7 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.37% of strains (540) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acetyltransf_9 | PF13527.14 | 1.4e-26 | 9–134 | Acetyltransferase (GNAT) domain |
Acetyltransf_17 | PF17668.8 | 1.4e-18 | 188–298 | Acetyltransferase (GNAT) domain |
SCP2_2 | PF13530.12 | 2.4e-15 | 301–395 | Sterol carrier protein domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3737 (transmembrane protein), medium confidence from genomic context alone (score 541 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2415c hyp |
hypothetical protein | 865 | 865 ctx | neighborhood:429 coexpression:774 |
Rv1258c tap |
multidrug-efflux transporter | 898 | 845 | coexpression:845 |
Rv1988 erm(37) |
23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) | 832 | 805 | coexpression:805 |
Rv3737 |
transmembrane protein | 540 | 541 ctx | cooccurence:475 |
Rv3365c hyp |
hypothetical protein | 521 | 521 ctx | cooccurence:518 |
Rv2417c |
DegV domain-containing protein | 691 | 499 ctx | neighborhood:494 textmining:410 |
Rv1226c |
transmembrane protein | 471 | 472 ctx | cooccurence:470 |
Rv2709 |
transmembrane protein | 442 | 442 ctx | cooccurence:442 |
Rv1249c |
membrane protein | 439 | 440 ctx | cooccurence:438 |
Rv2414c hyp |
hypothetical protein | 427 | 426 ctx | neighborhood:418 |
Rv2725c hflX |
GTP-binding protein HflX | 414 | 415 | coexpression:415 |
Rv3882c eccE1 |
ESX-1 secretion system protein EccE1 | 411 | 411 ctx | cooccurence:411 |
Rv1171 hyp |
hypothetical protein | 410 | 410 ctx | cooccurence:403 |
Rv0801 hyp |
hypothetical protein | 407 | 407 ctx | cooccurence:407 |
Rv0977 PE_PGRS16 |
PE-PGRS family protein PE_PGRS16 | 401 | 401 ctx | cooccurence:401 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): enhanced intracellular survival protein
- Pfam (hmmscan --cut_ga): Acetyltransf_9 PF13527.14 (E=1e-26), Acetyltransf_17 PF17668.8 (E=1e-18), SCP2_2 PF13530.12 (E=2e-15)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216932.2)
- Domains: Pfam-A via hmmscan --cut_ga — Acetyltransf_9 (PF13527.14), Acetyltransf_17 (PF17668.8), SCP2_2 (PF13530.12)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4552 - Curated reference: UniProt P9WFK7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
38 functional partner(s); context anchor
Rv3737 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2416c|eis MTVTLCSPTEDDWPGMFLLAAASFTDFIGPESATAWRTLVPTDGAVVVRDGAGPGSEVVGMALYMDLRLTVPGEVVLPTAGLSFVAVAPTHRRRGLLRAMCAELHRRIADSGYPVAALHASEGGIYGRFGYGPATTLHELTVDRRFARFHADAPGGGLGGSSVRLVRPTEHRGEFEAIYERWRQQVPGGLLRPQVLWDELLAECKAAPGGDRESFALLHPDGYALYRVDRTDLKLARVSELRAVTADAHCALWRALIGLDSMERISIITHPQDPLPHLLTDTRLARTTWRQDGLWLRIMNVPAALEARGYAHEVGEFSTVLEVSDGGRFALKIGDGRARCTPTDAAAEIEMDRDVLGSLYLGAHRASTLAAANRLRTKDSQLLRRLDAAFASDVPVQTAFEF