hsaG Resolved · high auto-curated
H37Rv Rv3535c · MTBC0 mtbc0_003752 ·
303 aa · 3997266–3998177 (-) ·
RefSeq NP_218052.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acetaldehyde dehydrogenase |
|---|---|
| MTBC0 PGAP re-annotation | acetaldehyde dehydrogenase (acetylating) |
| Revised (this work) | Acetaldehyde dehydrogenase (acetylating). Pfam: AcetDehyd-dimer (PF09290.17). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WQH3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Propanal dehydrogenase |
| EC (curated) |
EC 1.2.1.10, EC 1.2.1.87
|
| Curated function | Involved in cholesterol degradation. Catalyzes the conversion of propanal to propanoyl-CoA, using NAD(+) and coenzyme A. Has a broad substrate specificity, and can also use acetaldehyde, butyrlaldehyde, isobutyrlaldehyde and pentaldehyde as substrates. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
Q Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| Preferred name | hsaG |
| eggNOG description | Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD( ) and coenzyme A. Is the final enzyme in the meta- cleavage pathway for the degradation of aromatic compounds |
| Orthologous group | COG4569 |
| EC number |
EC 1.2.1.10
|
| KEGG orthology |
K04073
|
| KEGG pathways |
map00360, map00362, map00620, map00621, map00622, map00650, map01100, map01120, map01220
|
| KEGG modules |
M00545, M00569
|
| Gene Ontology (9) |
GO:0003674, GO:0003824, GO:0008150, GO:0008152, GO:0008774, GO:0016491, GO:0016620, GO:0016903, GO:0055114
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.348 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
AcetDehyd-dimer | PF09290.17 | 1.2e-60 | 127–272 | Prokaryotic acetaldehyde dehydrogenase, dimerisation |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: hsaF (4-hydroxy-2-oxovalerate aldolase), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3534c hsaF |
4-hydroxy-2-oxovalerate aldolase | 999 | 1000 ctx | neighborhood:881 cooccurence:774 coexpression:802 experimental:999 database:900 textmining:932 |
Rv3469c mhpE |
4-hydroxy-2-oxovalerate aldolase MhpE | 999 | 1000 ctx | cooccurence:774 experimental:987 database:900 |
Rv3536c hsaE |
hydratase | 999 | 996 ctx | neighborhood:879 cooccurence:772 coexpression:852 textmining:828 |
Rv3710 leuA |
2-isopropylmalate synthase | 985 | 984 | experimental:788 database:900 |
Rv3546 fadA5 |
acetyl-CoA acetyltransferase FadA | 936 | 906 | database:900 |
Rv0768 aldA |
aldehyde dehydrogenase AldA | 911 | 906 | database:900 |
Rv0223c |
aldehyde dehydrogenase | 909 | 905 | database:900 |
Rv0147 |
aldehyde dehydrogenase | 915 | 904 | database:900 |
Rv0458 |
aldehyde dehydrogenase | 913 | 904 | database:900 |
Rv3293 pcd |
piperideine-6-carboxylic acid dehydrogenase | 908 | 904 | database:900 |
Rv3523 ltp3 |
lipid carrier protein | 954 | 901 | database:900 textmining:563 |
Rv0859 fadA |
acyltransferase | 914 | 901 | database:900 |
Rv2501c accA1 |
acetyl/propionyl-CoA carboxylase subuit alpha | 905 | 901 | database:900 |
Rv2455c korA |
2-oxoglutarate oxidoreductase subunit KorA | 905 | 901 | database:900 |
Rv1867 hyp |
hypothetical protein | 905 | 901 | database:900 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: acetaldehyde dehydrogenase
- MTBC0 PGAP product: acetaldehyde dehydrogenase (acetylating)
- Pfam (hmmscan --cut_ga): AcetDehyd-dimer PF09290.17 (E=1e-60)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218052.1)
- Domains: Pfam-A via hmmscan --cut_ga — AcetDehyd-dimer (PF09290.17)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4569 - Curated reference: UniProt P9WQH3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
48 functional partner(s); context anchor
hsaF - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003752|Rv3535c|hsaG MPSKAKVAIVGSGNISTDLLYKLLRSEWLEPRWMVGIDPESDGLARAAKLGLETTHEGVDWLLAQPDKPDLVFEATSAYVHRDAAPKYAEAGIRAIDLTPAAVGPAVIPPANLREHLDAPNVNMITCGGQATIPIVYAVSRIVEVPYAEIVASVASVSAGPGTRANIDEFTKTTARGVQTIGGAARGKAIIILNPADPPMIMRDTIFCAIPTDADREAIAASIHDVVKEVQTYVPGYRLLNEPQFDEPSINSGGQALVTTFVEVEGAGDYLPPYAGNLDIMTAAATKVGEEIAKETLVVGGAR