Rv2622 Family assigned · medium auto-curated
H37Rv Rv2622 · MTBC0 mtbc0_002791 ·
273 aa · 2972198–2973019 (+) ·
RefSeq NP_217138.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | methyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | methyltransferase domain-containing protein |
| Revised (this work) | Methyltransferase domain-containing protein. Pfam: Methyltransf_25 (PF13649.13), Methyltransf_11 (PF08241.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6XES4
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Possible methyltransferase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
Q Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| eggNOG description | Methyltransferase |
| Orthologous group | COG0500 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.475 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Methyltransf_25 | PF13649.13 | 1.4e-09 | 58–150 | Methyltransferase domain |
Methyltransf_11 | PF08241.19 | 2.2e-10 | 59–154 | Methyltransferase domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv2621c (transcriptional regulator), medium confidence from genomic context alone (score 646 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2621c |
transcriptional regulator | 873 | 646 ctx | neighborhood:553 textmining:656 |
Rv2620c |
transmembrane protein | 615 | 615 ctx | neighborhood:552 |
Rv2619c hyp |
hypothetical protein | 574 | 574 ctx | neighborhood:553 |
Rv2623 TB31.7 |
universal stress protein | 490 | 490 ctx | neighborhood:487 |
Rv2033c hyp |
hypothetical protein | 874 | 71 | textmining:870 |
Rv1997 ctpF |
cation transporter ATPase F | 528 | 64 | textmining:517 |
Rv2036 hyp |
hypothetical protein | 518 | 55 | textmining:511 |
Rv1823 hyp |
hypothetical protein | 870 | 47 | textmining:870 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: methyltransferase
- MTBC0 PGAP product: methyltransferase domain-containing protein
- Pfam (hmmscan --cut_ga): Methyltransf_25 PF13649.13 (E=1e-09), Methyltransf_11 PF08241.19 (E=2e-10)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217138.1)
- Domains: Pfam-A via hmmscan --cut_ga — Methyltransf_25 (PF13649.13), Methyltransf_11 (PF08241.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0500 - Curated reference: UniProt I6XES4 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
8 functional partner(s); context anchor
Rv2621c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002791|Rv2622| MANKRGNAGQPLPLSDRDDDHMQGHWLLARLGKRVLRPGGVELTRTLLARAEVTDADVLELAPGLGRTAAEILARNPRSYVGAESDPNAANLVRHVLAGRGDVRVTDAADTGLSDASADVVIGEAMLTMQGNAAKHTIVAEAARVLRPGGRYAIHELALVPDDVAEQVRTDLRQSLARALKVNARPLTVAEWSHLLAGHGLVVEHVVTASMALLQPRRVIADEGLLGALRFAGNLLIHRAARRRVLLMRHTFRRHRERLTAVAIVAHKPHVDS