Rv2577 Family assigned · medium auto-curated
H37Rv Rv2577 · MTBC0 mtbc0_002744 ·
529 aa · 2924462–2926051 (+) ·
RefSeq NP_217093.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | metallophosphoesterase family protein |
| Revised (this work) | Metallophosphoesterase family protein. Pfam: Pur_ac_phosph_N (PF16656.12), Metallophos (PF00149.34). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WL81
SwissProt · reviewed
· Inferred from homology
|
|---|---|
| UniProt name | Uncharacterized protein Rv2577 |
UniProt still lists this protein as Uncharacterized protein Rv2577; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Calcineurin-like phosphoesterase |
| Orthologous group | COG1409 |
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.485 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 6 missense, 1 nonsense, 1 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 5.19% of strains (7534) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Pur_ac_phosph_N | PF16656.12 | 1.3e-15 | 68–148 | Purple acid Phosphatase, N-terminal domain |
Metallophos | PF00149.34 | 7.0e-11 | 211–389 | Calcineurin-like phosphoesterase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0090 (membrane protein), high confidence from genomic context alone (score 728 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0090 |
membrane protein | 728 | 728 ctx | cooccurence:713 |
Rv1619 hyp |
hypothetical protein | 565 | 565 ctx | cooccurence:565 |
Rv1006 hyp |
hypothetical protein | 557 | 558 ctx | cooccurence:556 |
Rv2576c |
membrane protein | 536 | 536 ctx | neighborhood:536 |
Rv0180c |
transmembrane protein | 487 | 488 ctx | cooccurence:478 |
Rv1868 hyp |
hypothetical protein | 488 | 481 ctx | cooccurence:471 |
Rv1372 pks18 |
alpha-pyrone synthesis polyketide synthase-like protein | 475 | 474 | coexpression:474 |
Rv1435c hyp |
hypothetical protein | 449 | 449 ctx | cooccurence:447 |
Rv3434c |
transmembrane protein | 446 | 446 ctx | cooccurence:446 |
Rv2874 dipZ |
integral membrane C-type cytochrome biogenesis protein DipZ | 457 | 438 ctx | cooccurence:413 |
Rv1871c hyp |
hypothetical protein | 431 | 431 ctx | cooccurence:431 |
Rv2598 hyp |
hypothetical protein | 418 | 418 ctx | cooccurence:415 |
Rv0178 |
Mce associated membrane protein | 441 | 417 | experimental:403 |
Rv1363c |
membrane protein | 438 | 413 | experimental:403 |
Rv1973 |
Mce associated membrane protein | 435 | 410 | experimental:403 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: metallophosphoesterase family protein
- Pfam (hmmscan --cut_ga): Pur_ac_phosph_N PF16656.12 (E=1e-15), Metallophos PF00149.34 (E=7e-11)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217093.1)
- Domains: Pfam-A via hmmscan --cut_ga — Pur_ac_phosph_N (PF16656.12), Metallophos (PF00149.34)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1409 - Curated reference: UniProt P9WL81 (SwissProt, reviewed; Inferred from homology)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
24 functional partner(s); context anchor
Rv0090 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002744|Rv2577| MGADLKQPQDADSPPKGVSRRRFLTTGAAAVVGTGVGAGGTALLSSHPRGPAVWYQRGRSGAPPVGGLHLQFGRNASTEMVVSWHTTDTVGNPRVMLGTPTSGFGSVVVAETRSYRDAKSNTEVRVNHAHLTNLTPDTDYVYAAVHDGTTPELGTARTAPSGRKPLRFTSFGDQSTPALGRLADGRYVSDNIGSPFAGDITIAIERIAPLFNLINGDLCYANLAQDRIRTWSDWFDNNTRSARYRPWMPAAGNHENEVGNGPIGYDAYQTYFAVPDSGSSPQLRGLWYSFTAGSVRVISLHNDDVCYQDGGNSYVRGYSGGEQRRWLQAELANARRDSEIDWVVVCMHQTAISTADDNNGADLGIRQEWLPLFDQYQVDLVVCGHEHHYERSHPLRGALGTDTRTPIPVDTRSDLIDSTRGTVHLVIGGGGTSKPTNALLFPQPRCQVITGVGDFDPAIRRKPSIFVLEDAPWSAFRDRDNPYGFVAFDVDPGQPGGTTSIKATYYAVTGPFGGLTVIDQFTLTKPRGG