Rv2571c Family assigned · medium auto-curated
H37Rv Rv2571c · MTBC0 mtbc0_002738 ·
355 aa · 2918437–2919504 (-) ·
RefSeq NP_217087.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane protein |
|---|---|
| MTBC0 PGAP re-annotation | aromatic acid exporter family protein |
| Revised (this work) | Aromatic acid exporter family protein. Pfam: FUSC_2 (PF13515.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WL89
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative arylamide transporter |
| Curated function | May be involved in the import of arylamide compounds. |
UniProt still lists this protein as Putative arylamide transporter; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Fusaric acid resistance protein-like |
| Orthologous group | COG4129 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 11 missense, 1 nonsense, 1 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.66% of strains (953) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
FUSC_2 | PF13515.13 | 4.0e-16 | 37–163 | Fusaric acid resistance protein-like |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: aspS (aspartate--tRNA ligase), high confidence from genomic context alone (score 848 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2572c aspS |
aspartate--tRNA ligase | 847 | 848 ctx | neighborhood:811 |
Rv1006 hyp |
hypothetical protein | 702 | 703 ctx | cooccurence:701 |
Rv1435c hyp |
hypothetical protein | 699 | 700 ctx | cooccurence:697 |
Rv1868 hyp |
hypothetical protein | 636 | 637 ctx | cooccurence:636 |
Rv3859c gltB |
glutamate synthase large subunit | 544 | 544 ctx | neighborhood:544 |
Rv0180c |
transmembrane protein | 534 | 534 ctx | cooccurence:534 |
Rv2598 hyp |
hypothetical protein | 524 | 524 ctx | cooccurence:522 |
Rv3134c |
universal stress protein | 495 | 496 ctx | cooccurence:493 |
Rv2082 hyp |
hypothetical protein | 493 | 493 ctx | cooccurence:489 |
Rv3033 hyp |
hypothetical protein | 488 | 488 ctx | cooccurence:488 |
Rv3448 eccD4 |
ESX-4 secretion system protein EccD4 | 473 | 473 ctx | cooccurence:473 |
Rv2047c hyp |
hypothetical protein | 463 | 464 ctx | cooccurence:459 |
Rv1748 hyp |
hypothetical protein | 458 | 459 ctx | cooccurence:457 |
Rv2597 |
membrane protein | 446 | 447 ctx | cooccurence:444 |
Rv3166c hyp |
hypothetical protein | 437 | 438 ctx | cooccurence:437 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane protein
- MTBC0 PGAP product: aromatic acid exporter family protein
- Pfam (hmmscan --cut_ga): FUSC_2 PF13515.13 (E=4e-16)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217087.1)
- Domains: Pfam-A via hmmscan --cut_ga — FUSC_2 (PF13515.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4129 - Curated reference: UniProt P9WL89 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
29 functional partner(s); context anchor
aspS - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002738|Rv2571c| MSASLLVRTACGGRAVAQRLRTVLWPITQTSVVAGLAWYLTHDVFNHPQAFFAPISAVVCMSATNVLRARRAQQMIVGVALGIVLGAGVHALLGSGPIAMGVVVFIALSVAVLCARGLVAQGLMFINQAAVSAVLVLVFASNGSVVFERLFDALVGGGLAIVFSILLFPPDPVVMLCSARADVLAAVRDILAELVNTVSDPTSAPPDWPMAAADRLHQQLNGLIEVRANAAMVARRAPRRWGVRSTVRDLDQQAVYLALLVSSVLHLARTIAGPGGDKLPTPVHAVLTDLAAGTGLADADPTAANEHAAAARATASTLQSAACGSNEVVRADIVQACVTDLQRVIERPGPSGMSA