Rv2030c Family assigned · medium auto-curated
H37Rv Rv2030c · MTBC0 mtbc0_002163 ·
681 aa · 2305054–2307099 (-) ·
RefSeq NP_216546.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | erythromycin esterase family protein |
| Revised (this work) | Erythromycin esterase family protein. Pfam: Pribosyltran (PF00156.34), Erythro_esteras (PF05139.20). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WLM1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized protein Rv2030c |
UniProt still lists this protein as Uncharacterized protein Rv2030c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | phosphoribosyl transferase |
| Orthologous group | COG1926 |
| KEGG orthology |
K07100
|
| Gene Ontology (13) |
GO:0005575, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0016020, GO:0030312, GO:0044424, GO:0044444, GO:0044464 +1 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.487 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 7 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 4.32% of strains (6267) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Pribosyltran | PF00156.34 | 5.7e-14 | 24–179 | Phosphoribosyl transferase domain |
Erythro_esteras | PF05139.20 | 2.2e-123 | 294–656 | Erythromycin esterase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pfkB (6-phosphofructokinase PfkB), high confidence from genomic context alone (score 984 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2029c pfkB |
6-phosphofructokinase PfkB | 996 | 984 ctx | neighborhood:865 coexpression:860 textmining:800 |
Rv2031c hspX |
alpha-crystallin | 980 | 966 ctx | neighborhood:792 coexpression:804 textmining:452 |
Rv2028c |
universal stress protein | 986 | 962 ctx | neighborhood:808 coexpression:810 textmining:665 |
Rv2032 acg |
NAD(P)H nitroreductase | 963 | 941 ctx | neighborhood:506 coexpression:860 textmining:407 |
Rv2625c rip3 |
zinc metalloprotease Rip3 | 949 | 913 ctx | cooccurence:498 coexpression:831 textmining:441 |
Rv2626c hrp1 |
hypoxic response protein | 939 | 901 | coexpression:860 textmining:412 |
Rv1997 ctpF |
cation transporter ATPase F | 892 | 871 | coexpression:815 |
Rv1737c narK2 |
nitrate/nitrite transporter | 896 | 860 | coexpression:860 |
Rv1733c |
transmembrane protein | 881 | 860 | coexpression:860 |
Rv2627c hyp |
hypothetical protein | 860 | 860 | coexpression:860 |
Rv1736c narX |
nitrate reductase-like protein NarX | 892 | 855 | coexpression:855 |
Rv3127 hyp |
hypothetical protein | 882 | 848 | coexpression:808 |
Rv3131 |
NAD(P)H nitroreductase | 919 | 841 | coexpression:801 textmining:514 |
Rv2623 TB31.7 |
universal stress protein | 878 | 839 | coexpression:833 |
Rv1996 |
universal stress protein | 852 | 836 | coexpression:802 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: erythromycin esterase family protein
- Pfam (hmmscan --cut_ga): Pribosyltran PF00156.34 (E=6e-14), Erythro_esteras PF05139.20 (E=2e-123)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216546.1)
- Domains: Pfam-A via hmmscan --cut_ga — Pribosyltran (PF00156.34), Erythro_esteras (PF05139.20)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1926 - Curated reference: UniProt P9WLM1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
39 functional partner(s); context anchor
pfkB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002163|Rv2030c| MLMTAAADVTRRSPRRVFRDRREAGRVLAELLAAYRDQPDVIVLGLARGGLPVAWEVAAALHAPLDAFVVRKLGAPGHDEFAVGALASGGRVVVNDDVVRGLRITPQQLRDIAEREGRELLRRESAYRGERPPTDITGKTVIVVDDGLATGASMFAAVQALRDAQPAQIVIAVPAAPESTCREFAGLVDDVVCATMPTPFLAVGESFWDFRQVTDEEVRRLLATPTAGPSLRRPAASTAADVLRRVAIDAPGGVPTHEVLAELVGDARIVLIGESSHGTHEFYQARAAMTQWLIEEKGFGAVAAEADWPDAYRVNRYVRGLGEDTNADEALSGFERFPAWMWRNTVVRDFVEWLRTRNQRYESGALRQAGFYGLDLYSLHRSIQEVISYLDKVDPRAAARARARYACFDHACADDGQAYGFAAAFGAGPSCEREAVEQLVDVQRNALAYARQDGLLAEDELFYAQQNAQTVRDAEVYYRAMFSGRVTSWNLRDQHMAQTLGSLLTHLDRHLDAPPARIVVWAHNSHVGDARATEVWADGQLTLGQIVRERYGDESRSIGFSTYTGTVTAASEWGGIAQRKAVRPALHGSVEELFHQTADSFLVSARLSRDAEAPLDVVRLGRAIGVVYLPATERQSHYLHVRPADQFDAMIHIDQTRALEPLEVTSRWIAGENPETYPTGL