katG Resolved · high auto-curated
H37Rv Rv1908c · MTBC0 mtbc0_002023 ·
740 aa · 2171999–2174221 (-) ·
RefSeq NP_216424.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | catalase-peroxidase |
|---|---|
| MTBC0 PGAP re-annotation | catalase/peroxidase HPI |
| Revised (this work) | Catalase/peroxidase HPI. Pfam: peroxidase (PF00141.29). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WIE5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Catalase-peroxidase |
| EC (curated) |
EC 1.11.1.21
|
| Curated function | Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity, oxidizing various electron donors including NADP(H). Protects M.tuberculosis against toxic reactive oxygen species (ROS) including hydrogen peroxide as well as organic peroxides and thus contributes to its survival within host macrophages by countering the phagocyte oxidative burst. Also displays efficient peroxynitritase activity, which may help the bacterium to persist in macrophages..; FUNCTION: Might be involved in DNA repair. Partly complements recA-deficient E.coli cells exposed to UV radiation, mitomycin C or. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | katG |
| eggNOG description | Bifunctional enzyme with both catalase and broad- spectrum peroxidase activity |
| Orthologous group | COG0376 |
| EC number |
EC 1.11.1.21
|
| KEGG orthology |
K03782
|
| KEGG pathways |
map00360, map00380, map00940, map00983, map01100, map01110
|
| Gene Ontology (130) |
GO:0000166, GO:0000302, GO:0003674, GO:0003824, GO:0004096, GO:0004601, GO:0005488, GO:0005575, GO:0005576, GO:0005618, GO:0005622, GO:0005623 +118 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.198 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 8 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
peroxidase | PF00141.29 | 3.7e-40 | 94–397 | Peroxidase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: furA (ferric uptake regulation protein FurA), high confidence from genomic context alone (score 837 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1907c hyp |
hypothetical protein | 957 | 945 ctx | neighborhood:805 coexpression:731 |
Rv3566c nat |
arylamine N-acetyltransferase | 912 | 901 | database:900 |
Rv1600 hisC1 |
histidinol-phosphate aminotransferase | 910 | 901 | database:900 |
Rv2231c cobC |
aminotransferase | 905 | 900 | database:900 |
Rv3772 hisC2 |
histidinol-phosphate aminotransferase | 905 | 900 | database:900 |
Rv1909c furA |
ferric uptake regulation protein FurA | 979 | 837 ctx | neighborhood:829 textmining:879 |
Rv3838c pheA |
prephenate dehydratase | 801 | 801 | database:800 |
Rv3846 sodA |
superoxide dismutase | 973 | 516 | database:500 textmining:948 |
Rv0432 sodC |
superoxide dismutase | 936 | 503 | database:500 textmining:878 |
Rv1910c hyp |
hypothetical protein | 541 | 471 ctx | neighborhood:471 |
Rv1906c hyp |
hypothetical protein | 508 | 423 ctx | neighborhood:415 |
Rv1905c aao |
D-amino acid oxidase | 418 | 395 | |
Rv3914 trxC |
thioredoxin TrxC | 578 | 387 | |
Rv1471 trxB1 |
thioredoxin | 455 | 366 | |
Rv1324 |
thioredoxin | 415 | 360 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: catalase-peroxidase
- MTBC0 PGAP product: catalase/peroxidase HPI
- Pfam (hmmscan --cut_ga): peroxidase PF00141.29 (E=4e-40)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216424.1)
- Domains: Pfam-A via hmmscan --cut_ga — peroxidase (PF00141.29)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0376 - Curated reference: UniProt P9WIE5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
71 functional partner(s); context anchor
furA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002023|Rv1908c|katG MPEQHPPITETTTGAASNGCPVVGHMKYPVEGGGNQDWWPNRLNLKVLHQNPAVADPMGAAFDYAAEVATIDVDALTRDIEEVMTTSQPWWPADYGHYGPLFIRMAWHAAGTYRIHDGRGGAGGGMQRFAPLNSWPDNASLDKARRLLWPVKKKYGKKLSWADLIVFAGNCALESMGFKTFGFGFGRVDQWEPDEVYWGKEATWLGDERYSGKRDLENPLAAVQMGLIYVNPEGPNGNPDPMAAAVDIRETFRRMAMNDVETAALIVGGHTFGKTHGAGPADLVGPEPEAAPLEQMGLGWKSSYGTGTGKDAITSGIEVVWTNTPTKWDNSFLEILYGYEWELTKSPAGAWQYTAKDGAGAGTIPDPFGGPGRSPTMLATDLSLRVDPIYERITRRWLEHPEELADEFAKAWYKLIHRDMGPVARYLGPLVPKQTLLWQDPVPAVSHDLVGEAEIASLKSQILASGLTVSQLVSTAWAAASSFRGSDKRGGANGGRIRLQPQVGWEVNDPDGDLRKVIRTLEEIQESFNSAAPGNIKVSFADLVVLGGCAAIEKAAKAAGHNITVPFTPGRTDASQEQTDVESFAVLEPKADGFRNYLGKGNPLPAEYMLLDKANLLTLSAPEMTVLVGGLRVLGANYKRLPLGVFTEASESLTNDFFVNLLDMGITWEPSPADDGTYQGKDGSGKVKWTGSRVDLVFGSNSELRALVEVYGADDAQPKFVQDFVAAWDKVMNLDRFDVR